Regulation of Gdnf expression by retinoic acid in Sertoli cells

Silvia Masciarelli, Rossana Saracino, Chiara Capponi, Sara Di Persio, Carla Boitani, Francesco Fazi, Stefania Fera, Elena Vicini

Risultato della ricerca: Contributo in rivistaArticolo in rivista

2 Citazioni (Scopus)

Abstract

Glial cell line-derived neurotrophic factor (GDNF) and retinoic acid (RA) are two molecules crucial for the regulation of the spermatogonial compartment of the testis. During the cycle of the seminiferous epithelium, their relative concentration oscillates with lower GDNF levels in stages where RA levels are high. It has been recently shown that RA negatively regulates Gdnf expression but the mechanisms behind are so far unknown. Here, we show that RA directly downregulates Gdnf mRNA levels in primary murine Sertoli cells through binding of RARα to a novel DR5-RARE on Gdnf promoter. Pharmacological inhibition and chromatin immunoprecipitation–quantitative polymerase chain reaction analysis suggested that the underlying mechanism involved histone deacetylase activity and epigenetic repression of Gdnf promoter upon RA treatment.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
RivistaMolecular Reproduction and Development
Volume2020
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • GDNF
  • Sertoli cells
  • cycle of the seminiferous epithelium
  • retinoic acid

Fingerprint

Entra nei temi di ricerca di 'Regulation of Gdnf expression by retinoic acid in Sertoli cells'. Insieme formano una fingerprint unica.

Cita questo