Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Pál Vági; Tito Caffi; Kálmán Zoltán Váczy; Márk Z. Németh; Levente Kiss

doi:10.1007/s10658-015-0797-2

Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Pál Vági
, Tito Caffi
, Kálmán Zoltán Váczy
, Márk Z. Németh
, Levente Kiss^*

^*Autore corrispondente per questo lavoro

Hungarian Academy of Sciences
Eötvös Loránd University
Eszterhazy Karoly University

Risultato della ricerca: Contributo in rivista › Articolo › peer review

3 Citazioni (Scopus)

Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

Lingua originale	Inglese
pagine (da-a)	799-802
Numero di pagine	4
Rivista	European Journal of Plant Pathology
Volume	144
Numero di pubblicazione	4
DOI	https://doi.org/10.1007/s10658-015-0797-2
Stato di pubblicazione	Pubblicato - 2016

All Science Journal Classification (ASJC) codes

Agronomia e Scienze della Produzione Vegetale
Botanica
Orticoltura

Keywords

Agronomy and Crop Science
Epifluorescence microscopy
Ethidium bromide
Fluorescein diacetate
Grapevine powdery mildew
Horticulture
Plant Science
Propidium iodide

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10.1007/s10658-015-0797-2

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title = "Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator",

abstract = "The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.",

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AU - Vági, Pál

AU - Caffi, Tito

AU - Váczy, Kálmán Zoltán

AU - Németh, Márk Z.

AU - Kiss, Levente

PY - 2016

Y1 - 2016

N2 - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

AB - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

KW - Agronomy and Crop Science

KW - Epifluorescence microscopy

KW - Ethidium bromide

KW - Fluorescein diacetate

KW - Grapevine powdery mildew

KW - Horticulture

KW - Plant Science

KW - Propidium iodide

KW - Agronomy and Crop Science

KW - Epifluorescence microscopy

KW - Ethidium bromide

KW - Fluorescein diacetate

KW - Grapevine powdery mildew

KW - Horticulture

KW - Plant Science

KW - Propidium iodide

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Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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