Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Tito Caffi, Pál Vági, Kálmán Zoltán Váczy, Márk Z. Németh, Levente Kiss

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

3 Citazioni (Scopus)

Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.
Lingua originaleEnglish
pagine (da-a)799-802
Numero di pagine4
RivistaEuropean Journal of Plant Pathology
Volume144
DOI
Stato di pubblicazionePubblicato - 2016

Keywords

  • Agronomy and Crop Science
  • Epifluorescence microscopy
  • Ethidium bromide
  • Fluorescein diacetate
  • Grapevine powdery mildew
  • Horticulture
  • Plant Science
  • Propidium iodide

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