Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Pál Vági; Tito Caffi; Kálmán Zoltán Váczy; Márk Z. Németh; Levente Kiss

doi:10.1007/s10658-015-0797-2

Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Pál Vági, Tito Caffi, Kálmán Zoltán Váczy, Márk Z. Németh, Levente Kiss

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

3 Citazioni (Scopus)

Abstract

The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

Lingua originale	English
pagine (da-a)	799-802
Numero di pagine	4
Rivista	European Journal of Plant Pathology
Volume	144
DOI	https://doi.org/10.1007/s10658-015-0797-2
Stato di pubblicazione	Pubblicato - 2016

Keywords

Agronomy and Crop Science
Epifluorescence microscopy
Ethidium bromide
Fluorescein diacetate
Grapevine powdery mildew
Horticulture
Plant Science
Propidium iodide

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10.1007/s10658-015-0797-2

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title = "Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator",

abstract = "The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.",

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doi = "10.1007/s10658-015-0797-2",

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T1 - Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

AU - Vági, Pál

AU - Caffi, Tito

AU - Váczy, Kálmán Zoltán

AU - Németh, Márk Z.

AU - Kiss, Levente

PY - 2016

Y1 - 2016

N2 - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

AB - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.

KW - Agronomy and Crop Science

KW - Epifluorescence microscopy

KW - Ethidium bromide

KW - Fluorescein diacetate

KW - Grapevine powdery mildew

KW - Horticulture

KW - Plant Science

KW - Propidium iodide

KW - Agronomy and Crop Science

KW - Epifluorescence microscopy

KW - Ethidium bromide

KW - Fluorescein diacetate

KW - Grapevine powdery mildew

KW - Horticulture

KW - Plant Science

KW - Propidium iodide

UR - http://hdl.handle.net/10807/95108

UR - http://www.wkap.nl/journalhome.htm/0929-1873

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DO - 10.1007/s10658-015-0797-2

M3 - Article

SN - 0929-1873

VL - 144

SP - 799

EP - 802

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

ER -

Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator

Abstract

Keywords

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