TY - JOUR
T1 - Refining a method for ascospore viability testing in overwintering chasmothecia of Erysiphe necator
AU - Vági, Pál
AU - Caffi, Tito
AU - Váczy, Kálmán Zoltán
AU - Németh, Márk Z.
AU - Kiss, Levente
PY - 2016
Y1 - 2016
N2 - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.
AB - The vital stain fluorescein diacetate (FDA) has long been used to assess the viability of Erysiphe necator ascospores in chasmothecia. To determine the viability of animal, plant and other cells, more complex staining procedures, for example simultaneous double-staining procedures based on the use of FDA and propidium iodide (PI), have also been widely used. This paper reports a refined, detailed version of this method and its limitations, where PI was replaced with ethidium bromide (EtBr), to be used in testing the viability of E. necator ascospores during overwintering in chasmothecia.
KW - Agronomy and Crop Science
KW - Epifluorescence microscopy
KW - Ethidium bromide
KW - Fluorescein diacetate
KW - Grapevine powdery mildew
KW - Horticulture
KW - Plant Science
KW - Propidium iodide
KW - Agronomy and Crop Science
KW - Epifluorescence microscopy
KW - Ethidium bromide
KW - Fluorescein diacetate
KW - Grapevine powdery mildew
KW - Horticulture
KW - Plant Science
KW - Propidium iodide
UR - http://hdl.handle.net/10807/95108
UR - http://www.wkap.nl/journalhome.htm/0929-1873
U2 - 10.1007/s10658-015-0797-2
DO - 10.1007/s10658-015-0797-2
M3 - Article
SN - 0929-1873
VL - 144
SP - 799
EP - 802
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
ER -