Reduced inhibitory effects of clonidine and neuropeptide Y on 3H-noradrenaline release from synaptosomes of the medulla oblongata of the spontaneously hypertensive rat

Maria Martire, Paolo Preziosi, K Fuxe, G Pistritto, Lf Agnati

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

The release of 3H-noradrenaline (3H-NA) evoked by high-K+ (15 mM) was studied in synaptosomes isolated from the medulla oblongata of the normotensive Wistar-Kyoto and spontaneously hypertensive male rat (14 weeks old) using a superfusion apparatus. Based on concentration-response curves clonidine was shown to have a reduced ability to inhibit 3H-NA release in synaptosomes isolated from the spontaneously hypertensive rat (SHR) versus the normotensive rat. Furthermore, only a high concentration of NPY (100 nM) had the ability to enhance the inhibitory effects of clonidine on 3H-NA release in synaptosomes isolated from the medulla oblongata of the SH male rat, while 1 nM of NPY was effective in synaptosomes isolated from the medulla oblongata of the normotensive Wistar-Kyoto rat. These results may indicate a reduced presynaptic alpha 2-adrenoceptor and NPY receptor function to inhibit 3H-NA release from NA and/or adrenaline (A) nerve terminals in the medulla oblongata of the adult 14 weeks old SHR.
Lingua originaleEnglish
pagine (da-a)181-189
Numero di pagine9
RivistaJournal of Neural Transmission
Volume76
Stato di pubblicazionePubblicato - 1989

Keywords

  • Animals
  • Clonidine
  • Male
  • Medulla Oblongata
  • Neuropeptide Y
  • Norepinephrine
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred Strains
  • Synaptosomes

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