redox regulation of cell proliferation by pyrrolidine dithiocarbamate in thymoma cells transplanted in vivo

Gabriella Calviello, Simona Serini, Alma Boninsegna Lucarelli, Paola Palozza

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

Pyrrolidine dithiocarbamate (PDTC) is a synthetic compound largely used in cell biological studies and known to exert either antioxidant or pro-oxidant effects. Recently, its antitumoral activity has been proposed on the basis of its antioxidant and proapoptotic effects. In the present study, we evaluated the effect of increasing i.p. doses of PDTC on the growth of a strain of highly malignant thymoma cells inoculated in the peritoneum of inbred Balb/c mice. PDTC treatment increased the number of thymoma cells in a dose-dependent manner, enhancing the percentage of proliferating tumor cells. PDTC exerted regulatory effects on cell cycle distribution, decreasing the expression of cell cycle inhibitors. Alterations in the production of intracellular reactive oxygen species, levels of oxidized glutathione, and intracellular levels of the redox-active metals iron and copper were also observed. The above results represent the first evidence that PDTC may induce in vivo cell proliferation in a murine thymoma cell model. In addition, we suggest that the ability of PDTC to bind and transport metals inside the cell and its pro-oxidant property may be factors underlying its effects on thymoma cell proliferation and cell cycle distribution.
Lingua originaleEnglish
pagine (da-a)1424-1431
Numero di pagine8
RivistaFREE RADICAL BIOLOGY & MEDICINE
Stato di pubblicazionePubblicato - 2001

Keywords

  • Cell proliferation
  • PTDC
  • Tumor cells

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