TY - JOUR
T1 - Reconstitution of aluminium and iron core in horse spleen apoferritin
AU - Ciasca, Gabriele
AU - Chiarpotto, M
AU - Campi, G
AU - Bocca, B
AU - Rodio, M
AU - Pino, A
AU - Ricci, A
AU - Poccia, N
AU - Rossi, C
AU - Alimonti, A
AU - Amenitsch, H
AU - Sole, P
AU - Bianconi, A.
PY - 2011
Y1 - 2011
N2 - This study investigates the uptake of iron and aluminium by apoferritin. In particular, we provide the first evidence that apoferritin is able to bind in vitro the physiological form of aluminium, Al(OH)4 −, to reach an Al/Fe atomic ratio of about 0.15. Mass spectrometry analysis shows that the Al content increases linearly as a function of Al concentration in solution. These findings provide a better understanding of the Al uptake in vivo, confirming that the metal content of ferritin depends on the metal bio-availability.
AB - This study investigates the uptake of iron and aluminium by apoferritin. In particular, we provide the first evidence that apoferritin is able to bind in vitro the physiological form of aluminium, Al(OH)4 −, to reach an Al/Fe atomic ratio of about 0.15. Mass spectrometry analysis shows that the Al content increases linearly as a function of Al concentration in solution. These findings provide a better understanding of the Al uptake in vivo, confirming that the metal content of ferritin depends on the metal bio-availability.
KW - Aluminium Metal toxicity Ferritin SAXS ICP-MS Nanomedicine
KW - Aluminium Metal toxicity Ferritin SAXS ICP-MS Nanomedicine
UR - http://hdl.handle.net/10807/43072
U2 - 10.1007/s11051-011-0294-2
DO - 10.1007/s11051-011-0294-2
M3 - Article
SN - 1388-0764
VL - 13
SP - 6149
EP - 6155
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
ER -