Recombinant BCG-Rv1767 amount determines, in vivo, antigen-specific T cells location, frequency, and protective outcome

Michela Sali, Giovanni Delogu, V Speranza, A Colone, R Cicconi, G Palmieri, D Giovannini, M Grassi, M Mattei, F Andreola, V Colizzi, F. Mariani

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

5 Citazioni (Scopus)

Abstract

One possibility to improve the efficacy of BCG vaccine against TB is to create a recombinant BCG (r-BCG), increasing the expression of mycobacterial antigens, to ameliorate the response to BCG. Here we describe a new r-BCG expressing the gene Rv1767, induced by Mycobacterium tuberculosis during its survival in human macrophages. The r-BCG elicited a specific T cells response in Balb/c mice higher than wt BCG. The r-BCG amount used to immunise mice determined diverse Th1/Th2 equilibriums, which was not the same in spleen and Lymph Nodes. Differences in cytokines production were found for IL-10, IL-4, TNF-alpha, and Arginase-1, which, in some conditions, resulted higher in r-BCG as compared to wt BCG-immunised mice. The immunisation with r-BCG-Rv1767 induced a lesser protective activity than wt BCG in a mouse model of TB. This reduction might likely be explained by the specific T cells phenotype and setting existing before MTB challenge, induced by either the single or the triple dose of r-BCG. The use of this model may help to highlight the capacity of different M. tuberculosis antigens to induce a protective immune response, actually not necessarily embodied by an increased frequency of Antigen-specific effector memory T cells.
Lingua originaleEnglish
pagine (da-a)150-159
Numero di pagine10
RivistaMicrobial Pathogenesis
Volume48
DOI
Stato di pubblicazionePubblicato - 2010

Keywords

  • Animals
  • Antigens, Bacterial
  • Arginase
  • BCG Vaccine
  • Humans
  • Interferon-gamma
  • Interleukin-10
  • Interleukin-4
  • Lymphoid Tissue
  • Mice
  • Mice, Inbred BALB C
  • Mycobacterium bovis
  • Mycobacterium tuberculosis
  • Nitric Oxide Synthase Type II
  • T-Lymphocytes
  • T-Lymphocytes, Helper-Inducer
  • Tuberculosis
  • Tumor Necrosis Factor-alpha
  • Vaccines, Synthetic

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