TY - JOUR
T1 - Real-time PCR for quantification of eleven individual Fusarium species in cereals
AU - Mogens, Nicolaisen
AU - Skaidrė, Supronienė
AU - Linda Kærgaard, Nielsen
AU - Lazzaro, Irene
AU - Niels Henrik, Spliid
AU - Annemarie Fejer, Justesen
PY - 2009
Y1 - 2009
N2 - Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed.
Quantification of biomass of Fusarium species is essential to understand the interactions of individual species
in disease development. In this study quantitative real-time PCR assays based on the elongation factor 1 α
(EF1α) gene for the 11 Fusarium species F. graminearum, F. culmorum, F. poae, F. langsethiae, F. sporotrichioides,
F. equiseti, F. tricinctum, F. avenaceum, F. verticillioides, F. subglutinans and F. proliferatum were developed and
tested on 24 wheat and 24 maize field samples. The assays were found to be specific and sensitive. Generally,
the results from the quantitative real-time PCR assays corresponded well with mycotoxin data of the field
samples.
AB - Contamination of cereals with Fusarium species is one of the major sources of mycotoxins in food and feed.
Quantification of biomass of Fusarium species is essential to understand the interactions of individual species
in disease development. In this study quantitative real-time PCR assays based on the elongation factor 1 α
(EF1α) gene for the 11 Fusarium species F. graminearum, F. culmorum, F. poae, F. langsethiae, F. sporotrichioides,
F. equiseti, F. tricinctum, F. avenaceum, F. verticillioides, F. subglutinans and F. proliferatum were developed and
tested on 24 wheat and 24 maize field samples. The assays were found to be specific and sensitive. Generally,
the results from the quantitative real-time PCR assays corresponded well with mycotoxin data of the field
samples.
KW - Fusarium
KW - Real-time PCR
KW - maize
KW - mycotoxin
KW - wheat
KW - Fusarium
KW - Real-time PCR
KW - maize
KW - mycotoxin
KW - wheat
UR - http://hdl.handle.net/10807/13469
U2 - 10.1016/j.mimet.2008.10.016
DO - 10.1016/j.mimet.2008.10.016
M3 - Article
SN - 0167-7012
SP - 234
EP - 240
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
ER -