Abstract
Effective Botrytis bunch rot (BBR) management requires the reduction of bunch trash colonization and
the sporulation potential by Botrytis cinerea at flowering and behind (Fedele et al., 2017). In the scope to
better understand BBR epidemiology and evaluate the efficacy of early-season disease control, colonization
rate of B. cinerea was assessed in grapevine bunch trash by means of real-time PCR (qPCR). The
qPCR assay was optimized to quantify B. cinerea DNA in the presence of Vitis vinifera DNA in bunch
trash. The fungal colonization rate, namely Pathogen Coefficient (PC) (Saito et al. 2013), was calculated
for an amount of B. cinerea conidia added into bunch trash. The PC value increased with the increase of
B. cinerea DNA in the presence of V. vinifera DNA. Besides, the PC in different bunch trash infection
categories estimated by qPCR were compared to plating method and microscopic counts to evaluate
the incidence of colonized bunch trash and the sporulation potential. The obtained results establish the
qPCR assay as a potential tool to examine the colonization rate of bunch trash. Hence, the PC was determined
in field samples to compare the effect of different fungicide treatments for BBR control. This
work provides a new method to be used in studies on B. cinerea in vineyards.
Lingua originale | English |
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Titolo della pubblicazione ospite | Abstracts book of International Congress on Grapevine and Wine Sciences |
Pagine | 102 |
Numero di pagine | 1 |
Stato di pubblicazione | Pubblicato - 2018 |
Evento | International Congress on Grapevine and Wine Sciences - Logroño Durata: 7 nov 2018 → 9 nov 2018 |
Convegno
Convegno | International Congress on Grapevine and Wine Sciences |
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Città | Logroño |
Periodo | 7/11/18 → 9/11/18 |
Keywords
- Botrytis bunch rot
- epidemiology