Reactive oxygen species mediate sevoflurane- and desflurane-induced preconditioning in isolated human right atria in vitro

Jean Luc Hanouz, Lan Zhu, Sandrine Lemoine, Charline Durand, Olivier Lepage, Massimo Massetti, André Khayat, Benoît Plaud, Jean Louis Gérard

Risultato della ricerca: Contributo in rivistaArticolo in rivista

31 Citazioni (Scopus)


BACKGROUND: We examined the role of reactive oxygen species (ROS) in sevoflurane- and desflurane-induced preconditioning on isolated human right atrial myocardium. METHODS: We recorded isometric contraction of human right atrial trabeculae suspended in an oxygenated Tyrode's solution (34 degrees C, stimulation frequency 1 Hz). In all groups, a 30-min hypoxic period was followed by 60 min of reoxygenation. Ten minutes before hypoxia reoxygenation, muscles were exposed to 5 min of sevoflurane 2% or desflurane 6%. In separate groups, the sevoflurane 2% (Sevo + N-(2-mercaptopropionyl)-glycine [MPG]) or desflurane 6% (Des + MPG) was administered in the presence of 0.1 mM MPG, a ROS scavenger. The effect of 0.1 mM MPG alone was tested. Recovery of force after a 60-min reoxygenation period was compared between groups (mean +/- sd). RESULTS: Preconditioning with sevoflurane 2% (85% +/- 4% of baseline) or desflurane 6% (86% +/- 7% of baseline) enhanced the recovery of the force of myocardial contraction after 60 min reoxygenation compared with the control group (53% +/- 11% of baseline, P < 0.001). This effect was abolished in the presence of MPG (56% +/- 12% of baseline for Sevo + MPG, 48% +/- 13% of baseline for Des + MPG). The effect of MPG alone on the recovery of force was not different from the control group (57% +/- 7% of baseline versus 53% +/- 11%; P = NS). CONCLUSIONS: In vitro, sevoflurane and desflurane preconditioned human myocardium against hypoxia through a ROS-dependent mechanism.
Lingua originaleEnglish
pagine (da-a)1534-1539
Numero di pagine6
RivistaAnesthesia and Analgesia
Stato di pubblicazionePubblicato - 2007


  • Adult
  • Aged
  • Atrial Appendage
  • Atrial Function, Right
  • Humans
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial
  • Isoflurane
  • Methyl Ethers
  • Middle Aged
  • Reactive Oxygen Species


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