Rapid and sensitive method for high-performance liquid chromatographic analysis of pterins in biological fluids

Alfredo Pontecorvi, Fabiola Moretti, I Antonozzi, C Carducci, L Vestri

Risultato della ricerca: Contributo in rivistaArticolo in rivista

9 Citazioni (Scopus)

Abstract

A rapid and sensitive high-performance liquid chromatographic (HPLC) method for the analysis of the most important urinary pterins is described. The method involves a preliminary sample oxidation to stabilize and convert pterins into their fluorescent forms and a purification by anion-exchange chromatography, followed by a short reversed-phase HPLC separation with fluorometric detection and quantitation of the different pterins. A complete HPLC analysis is accomplished in as little as 15 min. The sensitivity of the method allows the detection of as little as 20 pg of each pterin with a mean recovery greater than 99% for all pterins analysed. Reference values were obtained from 50 normal babies aged between 1 and 120 days. A significant correlation was found between urinary biopterin levels and the age of the babies (r = 0.445), while neopterin did not show any significant correlation with age. The "biopterin neopterin creatinine ratio" (BNCR index) was also significantly correlated with the age of the babies (r = 0.428). This rapid and sensitive method for pterin determination in biological fluids may be useful in the differential diagnosis of the various hyperphenylalaninemic conditions identified by neonatal mass screening programmes.
Lingua originaleEnglish
pagine (da-a)319-324
Numero di pagine6
RivistaJOURNAL OF CHROMATOGRAPHY A
Volume459
Stato di pubblicazionePubblicato - 1988

Keywords

  • Chromatography, High Pressure Liquid
  • Female
  • Humans
  • Infant, Newborn
  • Male
  • Pterins
  • Spectrometry, Fluorescence

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