TY - JOUR
T1 - Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method.
AU - Nocca, Giuseppina
AU - Calla', Cinzia Anna Maria
AU - SA, Santini
AU - Amalfitano, A
AU - Marigo, Luca
AU - DV, Rossetti
AU - Spagnuolo, G
AU - Cordaro, Massimo
PY - 2018
Y1 - 2018
N2 - A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.
AB - A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.
KW - 18-β-glycyrrhetinic acid
KW - HPLC
KW - 18-β-glycyrrhetinic acid
KW - HPLC
UR - https://publicatt.unicatt.it/handle/10807/128360
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85057403592&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85057403592&origin=inward
U2 - 10.1155/2018/5673186
DO - 10.1155/2018/5673186
M3 - Article
SN - 1687-8779
VL - 2018
SP - 1
EP - 5
JO - International Journal of Analytical Chemistry
JF - International Journal of Analytical Chemistry
IS - 2018
ER -