Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism

Andrea Urbani, Alfredo Pontecorvi, Giuseppe Grande, Antonella Giampietro, Massimo Castagnola, Domenico Milardi, Federica Vincenzoni, Francesca Mancini, Ferran Barrachina, Rafael Oliva

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

In the grey zone of testosterone levels between 8 and 12 nmol/L, the usefulness of therapy is controversial; as such, markers of tissue action of androgens may be helpful in adjusting clinical decisions. To better understand the effect of the hypothalamic-pituitary-testicular axis on male accessory secretion, we performed a proteomic quantitative analysis of seminal plasma in patients with secondary hypogonadism, before and after testosterone replacement therapy (TRT). Ten male patients with postsurgical hypogonadotrophic hypogonadism were enrolled in this study, and five of these patients were evaluated after testosterone treatment. Ten men with proven fertility were selected as a control group. An aliquot of seminal plasma from each individual was subjected to an in-solution digestion protocol and analyzed using an Ultimate 3000 RSLC-nano HPLC apparatus coupled to a LTQ Orbitrap Elite mass spectrometer. The label-free quantitative analysis was performed via Precursor Ions Area Detector Node. Eleven proteins were identified as decreased in hypogonadic patients versus controls, which are primarily included in hydrolase activity and protein binding activity. The comparison of the proteome before and after TRT comes about within the discovery of six increased proteins. This is the primary application of quantitative proteomics pointed to uncover a cluster of proteins reflecting an impairment not only of spermatogenesis but of the epididymal and prostate epithelial cell secretory function in male hypogonadism. The identified proteins might represent putative clinical markers valuable within the follow-up of patients with distinctive grades of male hypogonadism.
Lingua originaleEnglish
pagine (da-a)1-13
Numero di pagine13
RivistaJournal of Clinical Medicine
Volume8
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • accessory glands
  • hypogonadism
  • proteomics
  • seminal plasma
  • testosterone
  • testosterone deficiency

Fingerprint Entra nei temi di ricerca di 'Quantitative Analysis of the Seminal Plasma Proteome in Secondary Hypogonadism'. Insieme formano una fingerprint unica.

Cita questo