Quantification of botrytis cinerea in grapevine bunch trash by real-time PCR

Giorgia Fedele, Vittorio Rossi, Caterina Morcia, Valeria Terzi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

4 Citazioni (Scopus)

Abstract

Quantification of colonization of grape bunch trash by Botrytis cinerea is crucial for Botrytis bunch rot (BBR) control. A previously developed quantitative polymerase chain reaction (qPCR) method was adapted to quantify B. cinerea DNA in grape bunch trash, and a colonization coefficient (CC) was calculated as the ratio between the DNA concentrations of B. cinerea and of Vitis vinifera. CC values increased linearly with the number of conidia of B. cinerea or the quantity of mycelium of B. cinerea added to the bunch trash increased. CC values also increased linearly in bunch trash samples containing increasing percentages of B. cinerea-colonized bunch trash; in the latter samples, CC values were correlated with subsequent assessments of B. cinerea colonization of trash (as determined by plating on agar) and sporulation on the trash (as determined by spore counts after incubation in humid chambers). The qPCR assay was also validated using trash collected from bunches treated or not treated with fungicides in three vineyards in two seasons. CC values reflected the reduction in sporulation and in latent infections of mature berries caused by fungicide application. The qPCR assay enables rapid, specific, sensitive, and reliable quantification of the degree of colonization of bunch trash by B. cinerea, which makes it a useful tool for studies of the epidemiology and management of BBR.
Lingua originaleEnglish
pagine (da-a)1312-1319
Numero di pagine8
RivistaPhytopathology
Volume109
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Botrytis
  • Colonization rate
  • Fungicides
  • Gray mold
  • Plant Diseases
  • Polymerase Chain Reaction
  • Sporulation potential
  • Vitis

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