pSTAT1, pSTAT3, and T-bet expression in peripheral blood mononuclear cells from relapsing-remitting multiple sclerosis patients correlates with disease activity

Massimiliano Mirabella, Giovanni Frisullo, Viviana Nociti, Raffaele Iorio, Agata Katia Patanella, Cristina Sancricca, Anna Paola Batocchi, Francesco Angelucci, Marcella Caggiula, Pietro Attilio Tonali

Risultato della ricerca: Contributo in rivistaArticolo in rivista

104 Citazioni (Scopus)

Abstract

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system, and it is considered to be a T helper 1 (Th1) cell-mediated autoimmune disease. T-bet has been identified as a key transcription factor for the development of Th1 cells and the induction of interferon (IFN)-gamma production. T-bet is induced during T-cell activation by the IFN-gamma signal transducer and activator of transcription (STAT)-1 signalling pathway. In this study we found an up-regulation of T-bet and pSTAT1 in peripheral blood CD4+ and CD8+ T cells and monocytes from relapsing-remitting MS patients in relapse compared with patients in remission and with healthy subjects. The increased expression of pSTAT1 strongly correlated with T-bet expression in CD4+ and CD8+ cells and monocytes from patients in relapse and was associated with an increased production of IFN-gamma by peripheral blood mononuclear cells (PBMCs). pSTAT3 was also up-regulated in CD4+ and CD8+ cells and monocytes from patients in relapse and was associated with an increased production of interleukin (IL)-10 but not of IL-6. pSTAT1, pSTAT3, and T-bet expression strongly correlated with Gd-DTPA-enhanced lesions on brain and spinal cord magnetic resonance imaging. Our data show for the first time that there is an up-regulation of type 1 immunity-correlated transcription factors such as STAT1 and T-bet in peripheral blood subpopulations of MS patients in the active phase of disease. The evaluation of T-bet and pSTAT1 expression in peripheral blood CD4+, CD8+ T cells and monocytes could be used as a marker of disease activity in relapsing-remitting MS.
Lingua originaleEnglish
pagine (da-a)1027-1036
Numero di pagine10
RivistaJournal of Neuroscience Research
Volume84
DOI
Stato di pubblicazionePubblicato - 2006

Keywords

  • Multiple Sclerosis, Relapsing-Remitting
  • STAT1 Transcription Factor
  • STAT3 Transcription Factor

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