Proteomic study of pilocytic astrocytoma pediatric brain tumor intracystic fluid.

Ilaria Inserra, Massimo Castagnola, Federica Iavarone, Claudia Martelli, Luca Dangelo, Daniela Delfino, Diana Valeria Rossetti, Gianpiero Tamburrini, Luca Massimi, Massimo Caldarelli, Massimo Caldarelli, Irene Messana, Claudia Desiderio

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12 Citazioni (Scopus)


Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted complementary and support each other in contributing to a wide characterization of the protein and peptide content of the tumor fluid. Top-down approach allowed to identify several proteins and peptides involved in different biological activities together with the characterization of interesting proteoforms such as fibrinopeptide A and its truncated form, fibrinopeptide B, complement C3f fragments, β-thymosin peptides, ubiquitin, several apolipoproteins belonging to A and C families, apolipoprotein J and D, and cystatin C. Of particular interest resulted the identification of a N-terminal truncated cystatin C proteoform, likely involved in immune response mechanism modulations and the identification of oxidized and glycosylated apolipoproteins including disulfide bridge dimeric forms. The bottom-up approach confirmed some of the experimental data findings together with adding the characterization of high-molecular-mass proteins in the samples. These data could contribute to elucidate the molecular mechanisms involved in onset and progression of the disease and cyst development.
Lingua originaleEnglish
pagine (da-a)4594-4606
Numero di pagine13
RivistaJournal of Proteome Research
Stato di pubblicazionePubblicato - 2014


  • Proteomic
  • Salivary


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