Proteomic investigation of whole saliva in Wilson's disease

Federica Iavarone, Tiziana Cabras, Maria Teresa Sanna, Barbara Manconi, Massimo Castagnola, Irene Messana, Monica Sanna, Daniela Fanni, Luigi Demelia, Orazio Sorbello, Gavino Faa

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

15 Citazioni (Scopus)

Abstract

Wilson's disease is a rare inherited disorder of copper metabolism, manifesting hepatic, neurological and psychiatric symptoms. Early diagnosis is often unfeasible and a unique diagnostic test is currently inapplicable. We performed the qualitative/quantitative characterization of the salivary proteome/peptidome of 32 Wilson's disease patients by an integrated top-down/bottom-up approach. Patients exhibited significant higher levels of S100A9 and S100A8 proteoforms, and their oxidized forms with respect to controls. Oxidation occurred on methionine and tryptophan residues, and on the unique cysteine residue, in position 42 in S100A8, and 3 in S100A9, that generated glutathionylated, cysteinylated, sulfinic, sulfonic, and disulfide dimeric forms. Wilson's disease patient saliva showed high levels of two new fragments of the polymeric immunoglobulin receptor, and of α-defensins 2 and 4. Overall, the salivary proteome of Wilson's disease patients reflected oxidative stress and inflammatory conditions characteristic of the pathology, highlighting differences that could be useful clues of disease exacerbation.
Lingua originaleEnglish
pagine (da-a)154-163
Numero di pagine10
RivistaJOURNAL OF PROTEOMICS
Volume128
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • HPLC–ESI-MS
  • Human saliva
  • Oxidative stress
  • Proteomics
  • S100A8
  • S100A9
  • Wilson's disease
  • pIgR
  • α-Defensins

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