Proteomic insight into the mitigation of wheat root drought stress by arbuscular mycorrhizae

Luigi Lucini, Letizia Bernardo, Caterina Morcia, Fulvia Rizza, Valeria Terzi, Paolo Carletti, Roberta Ghizzoni, Franz W. Badeck

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

26 Citazioni (Scopus)

Abstract

Arbuscular mycorrhizal fungi (AMF) are plant growth promoters that ameliorate plant-water relations and the nutrient uptake of wheat. In this work, two cultivars of Triticum spp., a bread and a durum wheat, grown under drought stress and inoculated or not by AMF, are evaluated through a shotgun proteomic approach. The AMF association had beneficial effects as compared to non-mycorrhizal roots, in both bread and durum wheat. The beneficial symbiosis was confirmed by measuring morphological and physiological traits. In our work, we identified 50 statistically differential proteins in the bread wheat cultivar and 66 differential proteins in the durum wheat cultivar. The findings highlighted a modulation of proteins related to sugar metabolism, cell wall rearrangement, cytoskeletal organization and sulphur-containing proteins, as well as proteins related to plant stress responses. Among differentially expressed proteins both cultivars evidenced a decrease in sucrose:fructan 6-fructosyltransferas. In durum wheat oxylipin signalling pathway was involved with two proteins: increased 12-oxo-phytodienoic acid reductase and decreased jasmonate-induced protein, both related to the biosynthesis of jasmonic acid. Interactome analysis highlighted the possible involvement of ubiquitin although not evidenced among differentially expressed proteins. The AMF association helps wheat roots reducing the osmotic stress and maintaining cellular integrity. Biological significance Drought is one of the major constraints that plants must face in some areas of the world, associated to climate change, negatively affecting the worldwide plant productivity. The adoption of innovative agronomic protocols may represent a winning strategy in facing this challenge. The arbuscular mycorrhizal fungi (AMF) inoculation may represent a natural and sustainable way to mitigate the negative effects due to drought in several crop, ameliorating plant growth and development. Studies on the proteomic responses specific to AMF in drought-stressed plants will help clarify how mycorrhization elicits plant growth, nutrient uptake, and stress-tolerance responses. Such studies also offer the potential to find biological markers and genetic targets to be used during breeding for new drought-resistant varieties.
Lingua originaleEnglish
pagine (da-a)21-32
Numero di pagine12
RivistaJOURNAL OF PROTEOMICS
Volume169
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • Biochemistry
  • Biophysics
  • Drought stress
  • Glomus mosseae
  • Shotgun proteomics
  • Sustainable agriculture
  • Triticum spp

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