Abstract
We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema. [Figure not available: see fulltext.].
Lingua originale | English |
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pagine (da-a) | 840-850 |
Numero di pagine | 11 |
Rivista | Journal of Clinical Immunology |
Volume | 40 |
DOI | |
Stato di pubblicazione | Pubblicato - 2020 |
Keywords
- 2-dimensional electrophoresis
- Angioedema
- Human saliva
- Mass spectrometry
- Tandem mass spectrometry
- mass spectrometry
- proteomics