TY - JOUR
T1 - Proteomic Analysis of the Acid-Insoluble Fraction of Whole Saliva from Patients Affected by Different Forms of Non-histaminergic Angioedema
AU - Firinu, Davide
AU - Arba, Morena
AU - Vincenzoni, Federica
AU - Iavarone, Federica
AU - Costanzo, Giulia
AU - Costanzo, Giuseppe
AU - Cabras, Tiziana
AU - Castagnola, Massimo
AU - Messana, Irene
AU - Del Giacco, Stefano R.
AU - Sanna, Maria T.
PY - 2020
Y1 - 2020
N2 - We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema. [Figure not available: see fulltext.].
AB - We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema. [Figure not available: see fulltext.].
KW - 2-dimensional electrophoresis
KW - Angioedema
KW - Human saliva
KW - Mass spectrometry
KW - Tandem mass spectrometry
KW - mass spectrometry
KW - proteomics
KW - 2-dimensional electrophoresis
KW - Angioedema
KW - Human saliva
KW - Mass spectrometry
KW - Tandem mass spectrometry
KW - mass spectrometry
KW - proteomics
UR - http://hdl.handle.net/10807/178335
U2 - 10.1007/s10875-020-00802-w
DO - 10.1007/s10875-020-00802-w
M3 - Article
SN - 0271-9142
VL - 40
SP - 840
EP - 850
JO - Journal of Clinical Immunology
JF - Journal of Clinical Immunology
ER -