Proteomic Analysis of the Acid-Insoluble Fraction of Whole Saliva from Patients Affected by Different Forms of Non-histaminergic Angioedema

Federica Iavarone, Giuseppe Costanzo, Massimo Castagnola, Irene Messana, Davide Firinu, Morena Arba, Giulia Costanzo, Tiziana Cabras, Stefano R. Del Giacco, Maria T. Sanna

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

We analyzed by bidimensional electrophoresis the acid-insoluble fraction of saliva from three classes of angioedema patients and a healthy control group, highlighting significant variations of several normalized spot volumes. Characterization of the corresponding proteins was performed by in-gel tryptic digestion of the spots, followed by high-resolution HPLC-ESI-MS/MS analysis of tryptic mixtures. By this strategy, 16 differentially-expressed proteins among two or more groups were identified. We found higher concentration of proteins involved in immune response (interleukin-1 receptor antagonist and annexin A1), and of moonlighting proteins acting as plasminogen receptors (glyceraldehyde-3-phosphate dehydrogenase, α-enolase, and annexin A2) in patients affected by the idiopathic non-histaminergic or hereditary angioedema with unknown origin with respect to healthy controls. These data provide new information on the molecular basis of these less characterized types of angioedema. [Figure not available: see fulltext.].
Lingua originaleEnglish
pagine (da-a)840-850
Numero di pagine11
RivistaJournal of Clinical Immunology
Volume40
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • 2-dimensional electrophoresis
  • Angioedema
  • Human saliva
  • Mass spectrometry
  • Tandem mass spectrometry
  • mass spectrometry
  • proteomics

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