Abstract
In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.
| Lingua originale | Inglese |
|---|---|
| pagine (da-a) | 83-92 |
| Numero di pagine | 10 |
| Rivista | Helicobacter Pylori |
| Volume | 2283 |
| DOI | |
| Stato di pubblicazione | Pubblicato - 2021 |
Keywords
- 16S ribosomal DNA
- Bacteria
- DNA, Bacterial
- DNA, Ribosomal
- Feces
- Gastrointestinal Microbiome
- High-Throughput Nucleotide Sequencing
- Humans
- Microbial community
- Next-generation sequencing
- PCR amplification
- Phylogeny
- RNA, Ribosomal, 16S
- Sequence Analysis, DNA
- Sequence-based analysis
- Stool samples
- Targeted amplicon sequencing