Abstract

In this chapter, we provide a methodological description of the process to perform gastrointestinal (GIT) microbiota profiling on human stool samples. The process includes: (i) collection of feces, (ii) isolation of DNA from fecal community bacteria, (iii) selection of both 16S rDNA sequencing target and next-generation sequencing platform, and (iv) analysis and interpretation of sequence data. The process culminates into a comprehensive report on the GIT microbiota composition and structure that may translate into clinically actionable results.
Lingua originaleEnglish
pagine (da-a)83-92
Numero di pagine10
RivistaHelicobacter Pylori
Volume2283
DOI
Stato di pubblicazionePubblicato - 2021

Keywords

  • 16S ribosomal DNA
  • Bacteria
  • DNA, Bacterial
  • DNA, Ribosomal
  • Feces
  • Gastrointestinal Microbiome
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Microbial community
  • Next-generation sequencing
  • PCR amplification
  • Phylogeny
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA
  • Sequence-based analysis
  • Stool samples
  • Targeted amplicon sequencing

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