Abstract
A ‘suspect screening analysis’ method for grape metabolomics by ultra-high performance-liquid chromatography (UHPLC) and highresolution
quadrupole-time of flight (QTOF) mass spectrometry was recently developed. This method was applied to study grape
monoterpene glycosides, the main grape aroma precursors. Since standard compounds were not available, they were tentatively
identified by overlapping various analytical approaches, in agreement with the indications recommended in mass spectrometry
(MS)-based metabolomics. Accurate mass and isotopic pattern, MS/MS fragmentation, correlation between fragments observed
and putative structures and between liquid chromatography coupled with mass spectrometry (LC/MS) and gas chromatography/
mass spectrometry signals were studied. Seventeen monoterpene glycosides were identified without performing the hydrolytic artifacts
commonly used to study these compounds whichmay affect sample profile. This is the first time that a detailed study of these
aroma precursors has been carried out by direct LC/MS analysis.
Lingua originale | English |
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pagine (da-a) | 1214-1222 |
Numero di pagine | 9 |
Rivista | Journal of Mass Spectrometry |
Volume | 49 |
DOI | |
Stato di pubblicazione | Pubblicato - 2014 |
Keywords
- aroma precursors
- grape
- metabolomics
- monoterpene glycosides
- time-of-flight mass spectrometry