Pro-inflammatory activation of a new immortalized human microglia cell line

Pierluigi Navarra, Lucia Lisi, Marta Chiavari

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

2 Citazioni (Scopus)

Abstract

The characterization of human microglia has been hampered by poor availability of human cell sources. However, microglia is involved in the physiopathology of multiple sclerosis, Alzheimer’s disease, Parkinson’s disease, HIV dementia, retinal degenerative diseases, cancer, and many other conditions. Therefore, there is an important need to have experimental paradigms of human microglia characterized and usable to study the role of microglia in the different pathologies in which it is involved. In the present work, we carried out an extensive characterization of Immortalized Human Microglia—SV40 cell line (IMhu), marketed by Applied Biological Material. The functional response of IMhu to a large variety of stimuli was studied. In particular, we investigated morphology, mortality, and changes in the production of different cytokines and chemokines, both under basal conditions and after stimulation. Moreover, western blotting analysis was conducted on phospho-mTOR (Ser 2448) and downstream parameters, p-P70S6K and 4EBP1, in order to understand if IMhu can be used for evaluations of mTOR pathway. In conclusion, IMhu cells proved to be a useful experimental model to investigate the physiopathology of inflammatory disease that involved microglia cells, including pathological conditions that involved the mTOR pathway.
Lingua originaleEnglish
pagine (da-a)111-123
Numero di pagine13
RivistaBrain Sciences
Volume9
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Cytokines
  • Human microglia
  • M1 phenotype
  • Mtor

Fingerprint Entra nei temi di ricerca di 'Pro-inflammatory activation of a new immortalized human microglia cell line'. Insieme formano una fingerprint unica.

Cita questo