Presynaptic A2-adrenoceptors and neuropeptide Y Y2 receptors inhibit [3H]noradrenaline release from rat hypothalamic synaptosomes via different mechanisms

Maria Martire, Nadia Mores, G Pistritto, Lf Agnati, K. Fuxe

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

22 Citazioni (Scopus)

Abstract

Presynaptic receptors may reduce transmitter release with different mechanisms. Both the alpha 2-agonist, clonidine and the Y2-agonist, neuropeptide Y fragment 13-36 (NPY 13-36), induce a concentration-dependent inhibition of the 4-aminopyridine (4-AP)-evoked [3H]noradrenaline ([3H]NA) release from hypothalamic synaptosomes. Changes in alpha 2- and Y2-modulation of noradrenaline (NA) release were observed by lowering the calcium influx with the use of omega-conotoxin (omega-CgTx), a calcium-channel blocking agent. In these experimental conditions, clonidine was less active, whereas NPY 13-36 preserved its efficacy. It therefore seems possible that presynaptic alpha 2-adrenoceptors can primarily inhibit NA release by reducing calcium influx via voltage-sensitive calcium channels (VSCC), while Y2-receptors may inhibit the intracellular release process with a mechanism independent of the calcium entry.
Lingua originaleEnglish
pagine (da-a)9-12
Numero di pagine4
RivistaNeuroscience Letters
Volume188
Stato di pubblicazionePubblicato - 1995

Keywords

  • 4-Aminopyridine
  • Animals
  • Calcium Channel Blockers
  • Clonidine
  • Hypothalamus
  • Male
  • Mollusk Venoms
  • Neuropeptide Y
  • Norepinephrine
  • Peptide Fragments
  • Peptides
  • Presynaptic Terminals
  • Rats
  • Rats, Wistar
  • Receptors, Adrenergic, alpha-2
  • Receptors, Neuropeptide Y
  • Synaptosomes
  • Time Factors
  • omega-Conotoxin GVIA

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