Potentially neuroprotective gene modulation in an in vitro model of mild traumatic brain injury.

Valentina Di Pietro, Barbara Tavazzi, Angela Maria Amorini, David A. Hovda, Stefano Signoretti, Christopher C. Giza, Giuseppe Lazzarino, Giacomo Lazzarino, Roberto Vagnozzi, Antonio Belli

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

28 Citazioni (Scopus)

Abstract

In this study, we investigated the hypothesis that mild traumatic brain injury (mTBI) triggers a controlled gene program as an adaptive response finalized to neuroprotection, similar to that found in hibernators and in ischemic preconditioning. A stretch injury device was used to produce an equi-biaxial strain field in rat organotypic hippocampal slice cultures at a specified Lagrangian strain of 10 % and a constant strain rate of 20 s(-1). After 24 h from injury, propidium iodide staining, HPLC analysis of metabolites and microarray analysis of cDNA were performed to evaluate cell viability, cell energy state and gene expression, respectively. Compared to control cultures, 10 % stretch injured cultures showed no change in viability, but demonstrated a hypometabolic state (decreased ATP, ATP/ADP, and nicotinic coenzymes) and a peculiar pattern of gene modulation. The latter was characterized by downregulation of genes encoding for proteins of complexes I, III, and IV of the mitochondrial electron transport chain and of ATP synthase; downregulation of transcriptional and translational genes; downregulation and upregulation of genes controlling the synthesis of glutamate and GABA receptors, upregulation of calmodulin and calmodulin-binding proteins; proper modulation of genes encoding for proapoptotic and antiapoptotic proteins. These results support the hypothesis that, following mTBI, a hibernation-type response is activated in non-hibernating species. Unlike in hibernators and ischemic preconditioning, this adaptive gene programme, aimed at achieving maximal neuroprotection, is not triggered by decrease in oxygen availability. It seems rather activated to avoid increase in oxidative/nitrosative stress and apoptosis during a transient period of mitochondrial malfunctioning.
Lingua originaleEnglish
pagine (da-a)185-198
Numero di pagine14
RivistaMolecular and Cellular Biochemistry
Volume2013
DOI
Stato di pubblicazionePubblicato - 2013

Keywords

  • mild traumatic brain injury
  • neuroprotective genes

Fingerprint

Entra nei temi di ricerca di 'Potentially neuroprotective gene modulation in an in vitro model of mild traumatic brain injury.'. Insieme formano una fingerprint unica.

Cita questo