Polymeric substrates with tunable elasticity and nanoscopically controlled biomolecule presentation

Tobias Longin Haas, Daniel Aydin, Ilia Louban, Nadine Perschmann, Jacques Blümmel, Theobald Lohmüller, Elisabetta Ada Cavalcanti-Adam, Henning Walczak, Horst Kessler, Roberto Fiammengo, Joachim P. Spatz

Risultato della ricerca: Contributo in rivistaArticolo in rivista

57 Citazioni (Scopus)

Abstract

Despite tremendous progress in recent years, nanopatterning of hydrated polymeric systems such as hydrogels still represents a major challenge. Here, we employ block copolymer nanolithography to arrange gold nanoparticles on a solid template, followed by the transfer of the pattern to a polymeric hydrogel. In the next step, these nanoparticles serve as specific anchor points for active biomolecules. We demonstrate the engineering of poly(ethylene glycol) hydrogel surfaces with respect to elasticity, nanopatterning, and functionalization with biomolecules. For the first time, biomolecule arrangement on the nanometer scale and substrate stiffness can be varied independently from each other. Young's moduli, a measure of the compliance of the substrates, can be tuned over 4 orders of magnitude, including the values for all of the different tissues found in the human body. Structured hydrogels can be used to pattern any histidinetagged protein as exemplified for his-protein A as an acceptor for immunoglobulin. When cell-adhesion-promoting peptide cRGDfK is selectively coupled to gold nanoparticles, the surfaces provide cues for cell-surface interaction and allow for the study of the modulation of cellular adhesion by the mechanical properties of the environment. Therefore, these substrates represent a unique multipurpose platform for studying receptor/ligand interactions with adhering cells, mechanotransduction, and cell-adhesion- dependent signaling. © 2010 American Chemical Society.
Lingua originaleEnglish
pagine (da-a)15472-15480
Numero di pagine9
RivistaLangmuir
Volume26
DOI
Stato di pubblicazionePubblicato - 2010

Keywords

  • Cell Adhesion
  • Cells, Cultured
  • Condensed Matter Physics
  • Elasticity
  • Electrochemistry
  • Humans
  • Hydrogels
  • Materials Science (all)
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Particle Size
  • Polymers
  • Spectroscopy
  • Surfaces and Interfaces

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