Polymerase chain reaction and reverse cross blot hybridization assay for detection of mycobacterial DNA in lupus vulgaris

Maurizio Sanguinetti, Brunella Posteraro, Giovanni Fadda, L. M. Chinni, S. Ruggeri, D. B. Camaioni, T. Gobello

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5 Citazioni (Scopus)

Abstract

For 5 years, an 83-year-old man had been suffering from slightly itchy erythematous plaques with clearcut margins, located on his left thigh and on his right arm; in addition, on his right auricle there was an erythematous patch with yellowish shadings that had appeared about 3 years before and had progressively spread to the temporal-zygomatic region, the chin and the mandibular arch. These lesions were strongly suggestive of lupus vulgaris; however the conventional bacteriological examinations performed on the biopsy specimen from lesional skin were negative. A diagnosis of lupus vulgaris was achieved through the detection of the 16S rRNA gene of Mycobacterium tuberculosis in a skin biopsy of the patient by means of a polymerase chain reaction followed by a reverse cross blot hybridization, a method which allows the identification of different mycobacterial species in a single hybridization procedure.
Lingua originaleEnglish
pagine (da-a)293-296
Numero di pagine4
RivistaDermatology
Volume195
Stato di pubblicazionePubblicato - 1997

Keywords

  • Aged
  • Aged, 80 and over
  • DNA, Bacterial
  • Humans
  • Lupus Vulgaris
  • Male
  • Mycobacterium tuberculosis
  • Nucleic Acid Hybridization
  • Polymerase Chain Reaction

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