TY - JOUR
T1 - Platelet reactivity and endothelial function in children of patients with early acute myocardial infarction
AU - Lanza, Gaetano Antonio
AU - Scalone, Giancarla
AU - Barone, Lucy
AU - Infusino, Fabio
AU - Coviello, Ilaria
AU - Di Monaco, Antonio
AU - Delogu, Angelica Bibiana
AU - Battipaglia, Irma
AU - De Nisco, Alessia
AU - Sestito, Alfonso
AU - Romagnoli, Costantino
AU - Crea, Filippo
PY - 2011
Y1 - 2011
N2 - AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P < 0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P < 0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli
AB - AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the response to the combined stimuli (e.g. MPA, 23.1 ± 12 vs. 5.63 ± 8, P < 0.001; platelet PAC-1, 57.7 ± 47 vs. 13.2 ± 7, P < 0.001). Compared with Group 2, Group 1 children showed lower FMD (10.7 ± 3.1 vs. 8.0 ± 2.9, respectively; P 0.007). However, no significant association was found between FMD and platelet reactivity. ConclusionOur results show increased platelet reactivity in children of patients with early AMI; the finding was not significantly correlated with endothelial dysfunction, suggesting that other mechanisms are mainly involved in the enhanced platelet response to agonistic stimuli
KW - platelets
KW - platelets
UR - http://hdl.handle.net/10807/7293
U2 - 10.1093/eurheartj/ehr109
DO - 10.1093/eurheartj/ehr109
M3 - Article
SN - 0195-668X
SP - 2042
EP - 2049
JO - European Heart Journal
JF - European Heart Journal
ER -
