TY - JOUR
T1 - Phytocomplex influences antimicrobial and health properties of concentrated glycerine macerates
AU - Di Vito, Maura
AU - Gentile, M.
AU - Mattarelli, P.
AU - Barbanti, L.
AU - Micheli, L.
AU - Mazzuca, C.
AU - Garzoli, S.
AU - Titubante, M.
AU - Vitali, A.
AU - Cacaci, Margherita
AU - Sanguinetti, Maurizio
AU - Bugli, Francesca
PY - 2020
Y1 - 2020
N2 - The purpose of this study was to correlate the chemical composition of four commercial concentrated glycerine macerates (C-GMs), produced through the same extraction method, with their in vitro antimicrobial, antioxidant, and immunomodulatory properties, in order to evaluate their potential for healing upper airway diseases. C-GMs of Carpinus betulus (CB), Ficus carica (FC), Alnus glutinosa (AG) and Ribes nigrum (RN) were studied. The quality was evaluated using HPLC and IM-SPME/GC-MS systems; anti-oxidant and anti-microbial activities were assessed by the respective DPPH test, and micro-broth dilution test performed against 10 strains of Streptococcus pyogenes and 10 probiotic strains. ELISA and MTT tests were used to assess the immunomodulatory activity and the cytotoxicity of C-GMs, respectively. A significant correlation was found between the number of active compounds and the in vitro C-GMs effectiveness. Furthermore, the C-GMs of AG showed the best anti-microbial activity on pathological strains and, together with CB, the best anti-oxidant activity. The ELISA test exhibited a good immunomodulatory activity of RN. In vitro data support the integrated use of C-GMs of CB, AG, and RN in presence of airway diseases, and highlight the importance of standard procedures in cultivation, harvest and post-harvest treatments, as a premise for C-GMs with consistent characteristics.
AB - The purpose of this study was to correlate the chemical composition of four commercial concentrated glycerine macerates (C-GMs), produced through the same extraction method, with their in vitro antimicrobial, antioxidant, and immunomodulatory properties, in order to evaluate their potential for healing upper airway diseases. C-GMs of Carpinus betulus (CB), Ficus carica (FC), Alnus glutinosa (AG) and Ribes nigrum (RN) were studied. The quality was evaluated using HPLC and IM-SPME/GC-MS systems; anti-oxidant and anti-microbial activities were assessed by the respective DPPH test, and micro-broth dilution test performed against 10 strains of Streptococcus pyogenes and 10 probiotic strains. ELISA and MTT tests were used to assess the immunomodulatory activity and the cytotoxicity of C-GMs, respectively. A significant correlation was found between the number of active compounds and the in vitro C-GMs effectiveness. Furthermore, the C-GMs of AG showed the best anti-microbial activity on pathological strains and, together with CB, the best anti-oxidant activity. The ELISA test exhibited a good immunomodulatory activity of RN. In vitro data support the integrated use of C-GMs of CB, AG, and RN in presence of airway diseases, and highlight the importance of standard procedures in cultivation, harvest and post-harvest treatments, as a premise for C-GMs with consistent characteristics.
KW - Alnus glutinosa
KW - Carpinus betulus
KW - Ficus carica
KW - IM-SPME/GC-MS system
KW - Ribes nigrum
KW - Alnus glutinosa
KW - Carpinus betulus
KW - Ficus carica
KW - IM-SPME/GC-MS system
KW - Ribes nigrum
UR - https://publicatt.unicatt.it/handle/10807/205369
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85097319043&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85097319043&origin=inward
U2 - 10.3390/antibiotics9120858
DO - 10.3390/antibiotics9120858
M3 - Article
SN - 2079-6382
VL - 9
SP - 1
EP - 15
JO - Antibiotics
JF - Antibiotics
IS - 12
ER -