TY - JOUR
T1 - PHYSIOLOGICAL EFFECTS OF TEMPERATURE AND A HERBICIDE MIXTURE ON THE SOFT-SHELL CLAM MYA ARENARIA (MOLLUSCA, BIVALVIA)
AU - Capri, Ettore
AU - Sacchi, Angela
AU - Fusi, Marco
AU - Luna, Greco
AU - Jocelyne, Pellerin
AU - Florent, Garnerot
AU - Severine, Louis
AU - Michel, Fournier
AU - Dominique, Lapointe
AU - Patrice, Couture
PY - 2011
Y1 - 2011
N2 - The aim of the current study was to investigate effects of temperature and a mixture of herbicides on the physiological status of the bivalve Mya arenaria. Bivalves acclimated to two temperatures (7 and 188C) were exposed for 28 d to 0.01 mg/L of a pesticide
formulation containing dichlorophenoxyacetic acid (2,4-D), 2-(2-methyl-4-chlorophenoxy) propionic acid (mecoprop), and 3,6- dichloro-2-methoxybenzoic acid (dicamba). At days 7, 14, and 28, mortality, immune parameters (hemocyte number, phagocytic activity, and efficiency), biomarkers of oxidative stress (catalase [CAT] and superoxide dismutase [SOD] activities and malondialdehyde[MDA] content), the metabolic enzyme cytochrome C oxidase (CCO), a biomarker of pesticide exposure (acetylcholinesterase [AChE]), and the activity of an enzyme related to gametogenesis (aspartate transcarbamylase [ATCase]) were monitored in clam tissues.
Gonadosomatic index (GSI), condition factor (CF), and sex were also assessed. In clams acclimated to 78C, exposure to pesticide enhanced CCO activity and CF and decreased MDA content, hemocyte number, CAT, and SOD activities. In clams kept at 188C,pesticide effects appeared minor compared with samples kept at 78C. In bivalves acclimated to 188C, CCO, SOD, and ATCase activity and MDA content were enhanced, and hemocyte number, CAT, and AchE activities and phagocytosis were suppressed. In samples exposed to pesticides, increased temperature enhanced MDA content and CCO and SOD activity and suppressed hemocyte number and CAT and AchE activity. A gradual sexual maturation was observed in both sexes through experimental time, but females had a higher sensitivity to temperature and pesticides compared to males. Increased temperature altered the ability of the sentinel species Mya arenaria to respond to pesticide exposures. Further work is needed to understand the impacts of increasing temperature on
the whole St. Lawrence estuary ecosystem.
AB - The aim of the current study was to investigate effects of temperature and a mixture of herbicides on the physiological status of the bivalve Mya arenaria. Bivalves acclimated to two temperatures (7 and 188C) were exposed for 28 d to 0.01 mg/L of a pesticide
formulation containing dichlorophenoxyacetic acid (2,4-D), 2-(2-methyl-4-chlorophenoxy) propionic acid (mecoprop), and 3,6- dichloro-2-methoxybenzoic acid (dicamba). At days 7, 14, and 28, mortality, immune parameters (hemocyte number, phagocytic activity, and efficiency), biomarkers of oxidative stress (catalase [CAT] and superoxide dismutase [SOD] activities and malondialdehyde[MDA] content), the metabolic enzyme cytochrome C oxidase (CCO), a biomarker of pesticide exposure (acetylcholinesterase [AChE]), and the activity of an enzyme related to gametogenesis (aspartate transcarbamylase [ATCase]) were monitored in clam tissues.
Gonadosomatic index (GSI), condition factor (CF), and sex were also assessed. In clams acclimated to 78C, exposure to pesticide enhanced CCO activity and CF and decreased MDA content, hemocyte number, CAT, and SOD activities. In clams kept at 188C,pesticide effects appeared minor compared with samples kept at 78C. In bivalves acclimated to 188C, CCO, SOD, and ATCase activity and MDA content were enhanced, and hemocyte number, CAT, and AchE activities and phagocytosis were suppressed. In samples exposed to pesticides, increased temperature enhanced MDA content and CCO and SOD activity and suppressed hemocyte number and CAT and AchE activity. A gradual sexual maturation was observed in both sexes through experimental time, but females had a higher sensitivity to temperature and pesticides compared to males. Increased temperature altered the ability of the sentinel species Mya arenaria to respond to pesticide exposures. Further work is needed to understand the impacts of increasing temperature on
the whole St. Lawrence estuary ecosystem.
KW - Chlorophenoxy herbicides
KW - Climate change
KW - Oxidative stress
KW - Sexual development
KW - Chlorophenoxy herbicides
KW - Climate change
KW - Oxidative stress
KW - Sexual development
UR - http://hdl.handle.net/10807/1910
M3 - Article
VL - 2010
SP - 132
EP - 141
JO - Environmental Toxicology and Chemistry
JF - Environmental Toxicology and Chemistry
SN - 0730-7268
ER -