Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

V Svicher; C Alteri; M Montano; R D'Arrigo; M Andreoni; G Angarano; A Antinori; G Antonelli; T Allice; P Bagnarelli; F Baldanti; A Bertoli; M Borderi; E Boeri; I Bon; B Bruzzone; Ap Callegaro; Capobianchi; G Carosi; Roberto Cauda; F Ceccherini Silberstein; M Clementi; A Chirianni; Manuela Colafigli; A D'Arminio Monforte; Andrea De Luca; A Di Biagio; G Di Nicuolo; G Di Perri; M Di Pietro; F Di Santo; L Fabeni; Giovanni Fadda; M Galli; W Gennari; V Ghisetti; A Giacometti; C Gori; A Gori; R Gulminetti; F Leoncini; G Maffongelli; F Maggiolo; G Manca; F Gargiulo; C Martinelli; R Maserati; F Mazzotta; G Meini; V Micheli; L Monno; C Mussini; P Narciso; S Nozza; S Paolucci; G Pal; S Parisi; G Parruti; Ar Pignataro; M Pollicita; T Quirino; Mc Re; G Rizzardini; Rosaria Santangelo; R Scaggiante; G Sterrantino; O Turriziani; Ml Vatteroni; L Vecchi; C Viscoli; V Vullo; M Zazzi; A Lazzarini; Cf Perno

Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

V Svicher, C Alteri, M Montano, R D'Arrigo, M Andreoni, G Angarano, A Antinori, G Antonelli, T Allice, P Bagnarelli, F Baldanti, A Bertoli, M Borderi, E Boeri, I Bon, B Bruzzone, Ap Callegaro, Capobianchi, G Carosi, Roberto CaudaF Ceccherini Silberstein, M Clementi, A Chirianni, Manuela Colafigli, A D'Arminio Monforte, Andrea De Luca, A Di Biagio, G Di Nicuolo, G Di Perri, M Di Pietro, F Di Santo, L Fabeni, Giovanni Fadda, M Galli, W Gennari, V Ghisetti, A Giacometti, C Gori, A Gori, R Gulminetti, F Leoncini, G Maffongelli, F Maggiolo, G Manca, F Gargiulo, C Martinelli, R Maserati, F Mazzotta, G Meini, V Micheli, L Monno, C Mussini, P Narciso, S Nozza, S Paolucci, G Pal, S Parisi, G Parruti, Ar Pignataro, M Pollicita, T Quirino, Mc Re, G Rizzardini, Rosaria Santangelo, R Scaggiante, G Sterrantino, O Turriziani, Ml Vatteroni, L Vecchi, C Viscoli, V Vullo, M Zazzi, A Lazzarini, Cf Perno^*

^*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivista › Articolo › peer review

22 Citazioni (Scopus)

Abstract

OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory.\r\n\r\nMETHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004).\r\n\r\nRESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM.\r\n\r\nCONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

Lingua originale	Inglese
pagine (da-a)	17-25
Numero di pagine	9
Rivista	New Microbiologica
Volume	35
Numero di pubblicazione	1
Stato di pubblicazione	Pubblicato - 2012

All Science Journal Classification (ASJC) codes

Medicina Generale

Keywords

Genotype
Genotyping Techniques
HIV Envelope Protein gp120
HIV Infections
HIV-1
Humans
Leukocytes
Mononuclear
Proviruses
Reproducibility of Results
Viral Load
Viral Tropism

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Svicher, V., Alteri, C., Montano, M., D'Arrigo, R., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Allice, T., Bagnarelli, P., Baldanti, F., Bertoli, A., Borderi, M., Boeri, E., Bon, I., Bruzzone, B., Callegaro, A., Capobianchi, Carosi, G., ... Perno, C. (2012). Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group. New Microbiologica, 35(1), 17-25.

@article{ce041a95412c462487f699327307de4b,

title = "Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group",

abstract = "OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory.\r\n\r\nMETHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004).\r\n\r\nRESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM.\r\n\r\nCONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.",

keywords = "Genotype, Genotyping Techniques, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Leukocytes, Mononuclear, Proviruses, Reproducibility of Results, Viral Load, Viral Tropism, Genotype, Genotyping Techniques, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Leukocytes, Mononuclear, Proviruses, Reproducibility of Results, Viral Load, Viral Tropism",

author = "V Svicher and C Alteri and M Montano and R D'Arrigo and M Andreoni and G Angarano and A Antinori and G Antonelli and T Allice and P Bagnarelli and F Baldanti and A Bertoli and M Borderi and E Boeri and I Bon and B Bruzzone and Ap Callegaro and Capobianchi and G Carosi and Roberto Cauda and {Ceccherini Silberstein}, F and M Clementi and A Chirianni and Manuela Colafigli and {D'Arminio Monforte}, A and {De Luca}, Andrea and {Di Biagio}, A and {Di Nicuolo}, G and {Di Perri}, G and {Di Pietro}, M and {Di Santo}, F and L Fabeni and Giovanni Fadda and M Galli and W Gennari and V Ghisetti and A Giacometti and C Gori and A Gori and R Gulminetti and F Leoncini and G Maffongelli and F Maggiolo and G Manca and F Gargiulo and C Martinelli and R Maserati and F Mazzotta and G Meini and V Micheli and L Monno and C Mussini and P Narciso and S Nozza and S Paolucci and G Pal and S Parisi and G Parruti and Ar Pignataro and M Pollicita and T Quirino and Mc Re and G Rizzardini and Rosaria Santangelo and R Scaggiante and G Sterrantino and O Turriziani and Ml Vatteroni and L Vecchi and C Viscoli and V Vullo and M Zazzi and A Lazzarini and Cf Perno",

year = "2012",

language = "English",

volume = "35",

pages = "17--25",

journal = "New Microbiologica",

issn = "1121-7138",

publisher = "Luigi Ponzio e figlio Editori",

number = "1",

}

Svicher, V, Alteri, C, Montano, M, D'Arrigo, R, Andreoni, M, Angarano, G, Antinori, A, Antonelli, G, Allice, T, Bagnarelli, P, Baldanti, F, Bertoli, A, Borderi, M, Boeri, E, Bon, I, Bruzzone, B, Callegaro, A, Capobianchi, Carosi, G, Cauda, R, Ceccherini Silberstein, F, Clementi, M, Chirianni, A, Colafigli, M, D'Arminio Monforte, A, De Luca, A, Di Biagio, A, Di Nicuolo, G, Di Perri, G, Di Pietro, M, Di Santo, F, Fabeni, L, Fadda, G, Galli, M, Gennari, W, Ghisetti, V, Giacometti, A, Gori, C, Gori, A, Gulminetti, R, Leoncini, F, Maffongelli, G, Maggiolo, F, Manca, G, Gargiulo, F, Martinelli, C, Maserati, R, Mazzotta, F, Meini, G, Micheli, V, Monno, L, Mussini, C, Narciso, P, Nozza, S, Paolucci, S, Pal, G, Parisi, S, Parruti, G, Pignataro, A, Pollicita, M, Quirino, T, Re, M, Rizzardini, G, Santangelo, R, Scaggiante, R, Sterrantino, G, Turriziani, O, Vatteroni, M, Vecchi, L, Viscoli, C, Vullo, V, Zazzi, M, Lazzarini, A & Perno, C 2012, 'Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group', New Microbiologica, vol. 35, n. 1, pagg. 17-25.

TY - JOUR

T1 - Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

AU - Svicher, V

AU - Alteri, C

AU - Montano, M

AU - D'Arrigo, R

AU - Andreoni, M

AU - Angarano, G

AU - Antinori, A

AU - Antonelli, G

AU - Allice, T

AU - Bagnarelli, P

AU - Baldanti, F

AU - Bertoli, A

AU - Borderi, M

AU - Boeri, E

AU - Bon, I

AU - Bruzzone, B

AU - Callegaro, Ap

AU - Capobianchi,

AU - Carosi, G

AU - Cauda, Roberto

AU - Ceccherini Silberstein, F

AU - Clementi, M

AU - Chirianni, A

AU - Colafigli, Manuela

AU - D'Arminio Monforte, A

AU - De Luca, Andrea

AU - Di Biagio, A

AU - Di Nicuolo, G

AU - Di Perri, G

AU - Di Pietro, M

AU - Di Santo, F

AU - Fabeni, L

AU - Fadda, Giovanni

AU - Galli, M

AU - Gennari, W

AU - Ghisetti, V

AU - Giacometti, A

AU - Gori, C

AU - Gori, A

AU - Gulminetti, R

AU - Leoncini, F

AU - Maffongelli, G

AU - Maggiolo, F

AU - Manca, G

AU - Gargiulo, F

AU - Martinelli, C

AU - Maserati, R

AU - Mazzotta, F

AU - Meini, G

AU - Micheli, V

AU - Monno, L

AU - Mussini, C

AU - Narciso, P

AU - Nozza, S

AU - Paolucci, S

AU - Pal, G

AU - Parisi, S

AU - Parruti, G

AU - Pignataro, Ar

AU - Pollicita, M

AU - Quirino, T

AU - Re, Mc

AU - Rizzardini, G

AU - Santangelo, Rosaria

AU - Scaggiante, R

AU - Sterrantino, G

AU - Turriziani, O

AU - Vatteroni, Ml

AU - Vecchi, L

AU - Viscoli, C

AU - Vullo, V

AU - Zazzi, M

AU - Lazzarini, A

AU - Perno, Cf

PY - 2012

Y1 - 2012

N2 - OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory.\r\n\r\nMETHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004).\r\n\r\nRESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM.\r\n\r\nCONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

AB - OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory.\r\n\r\nMETHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004).\r\n\r\nRESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM.\r\n\r\nCONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

KW - Genotype

KW - Genotyping Techniques

KW - HIV Envelope Protein gp120

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Leukocytes

KW - Mononuclear

KW - Proviruses

KW - Reproducibility of Results

KW - Viral Load

KW - Viral Tropism

KW - Genotype

KW - Genotyping Techniques

KW - HIV Envelope Protein gp120

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Leukocytes

KW - Mononuclear

KW - Proviruses

KW - Reproducibility of Results

KW - Viral Load

KW - Viral Tropism

UR - https://publicatt.unicatt.it/handle/10807/6319

UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84856910552&origin=inward

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84856910552&origin=inward

M3 - Article

SN - 1121-7138

VL - 35

SP - 17

EP - 25

JO - New Microbiologica

JF - New Microbiologica

IS - 1

ER -

Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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