Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

Roberto Cauda; Rosaria Santangelo; Manuela Colafigli; Andrea De Luca; Giovanni Fadda; Valentina Svicher; Claudia Alteri; Marco Montano; Roberta D'Arrigo; Massimo Andreoni; Gioacchino Angarano; Andrea Antinori; Guido Antonelli; Tiziano Allice; Patrizia Bagnarelli; Fausto Baldanti; Ada Bertoli; Marco Borderi; Enzo Boeri; Isabella Bon; Bianca Bruzzone; Anna Paola Callegaro; Maria Rosaria Capobianchi; Giampiero Carosi; Francesca Ceccherini-Silberstein; Massimo Clementi; Antonio Chirianni; Antonella D'Arminio Monforte; Antonio Di Biagio; Giuseppe Di Nicuolo; Giovanni Di Perri; Massimo Di Pietro; Fabiola Di Santo; Lavinia Fabeni; Massimo Galli; William Gennari; Valeria Ghisetti; Andrea Giacometti; Caterina Gori; Andrea Gori; Roberto Gulminetti; Francesco Leoncini; Gaetano Maffongelli; Franco Maggiolo; Giuseppe Manca; Franco Gargiulo; Canio Martinelli; Renato Maserati; Francesco Mazzotta; Genny Meini; Valeria Micheli; Laura Monno; Cristina Mussini; Pasquale Narciso; Silvia Nozza; Stefania Paolucci; Giorgio Palù; Saverio Parisi; Giustino Parruti; Angela Rosa Pignataro; Michela Pollicita; Tiziana Quirino; Maria Re Carla; Giuliano Rizzardini; Renzo Scaggiante; Gaetana Sterrantino; Ombretta Turriziani; Maria Linda Vatteroni; Laura Vecchi; Claudio Viscoli; Vincenzo Vullo; Maurizio Zazzi; Adriano Lazzarin; Carlo Federico Perno

Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

Roberto Cauda, Rosaria Santangelo, Manuela Colafigli, Andrea De Luca, Giovanni Fadda, Valentina Svicher, Claudia Alteri, Marco Montano, Roberta D'Arrigo, Massimo Andreoni, Gioacchino Angarano, Andrea Antinori, Guido Antonelli, Tiziano Allice, Patrizia Bagnarelli, Fausto Baldanti, Ada Bertoli, Marco Borderi, Enzo Boeri, Isabella BonBianca Bruzzone, Anna Paola Callegaro, Maria Rosaria Capobianchi, Giampiero Carosi, Francesca Ceccherini-Silberstein, Massimo Clementi, Antonio Chirianni, Antonella D'Arminio Monforte, Antonio Di Biagio, Giuseppe Di Nicuolo, Giovanni Di Perri, Massimo Di Pietro, Fabiola Di Santo, Lavinia Fabeni, Massimo Galli, William Gennari, Valeria Ghisetti, Andrea Giacometti, Caterina Gori, Andrea Gori, Roberto Gulminetti, Francesco Leoncini, Gaetano Maffongelli, Franco Maggiolo, Giuseppe Manca, Franco Gargiulo, Canio Martinelli, Renato Maserati, Francesco Mazzotta, Genny Meini, Valeria Micheli, Laura Monno, Cristina Mussini, Pasquale Narciso, Silvia Nozza, Stefania Paolucci, Giorgio Palù, Saverio Parisi, Giustino Parruti, Angela Rosa Pignataro, Michela Pollicita, Tiziana Quirino, Maria Re Carla, Giuliano Rizzardini, Renzo Scaggiante, Gaetana Sterrantino, Ombretta Turriziani, Maria Linda Vatteroni, Laura Vecchi, Claudio Viscoli, Vincenzo Vullo, Maurizio Zazzi, Adriano Lazzarin, Carlo Federico Perno

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

22 Citazioni (Scopus)

Abstract

OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory. METHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004). RESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM. CONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

Lingua originale	English
pagine (da-a)	17-25
Numero di pagine	9
Rivista	New Microbiologica
Volume	35
Stato di pubblicazione	Pubblicato - 2012

Keywords

Genotype
Genotyping Techniques
HIV Envelope Protein gp120
HIV Infections
HIV-1
Humans
Leukocytes, Mononuclear
Proviruses
Reproducibility of Results
Viral Load
Viral Tropism

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Cauda, R., Santangelo, R., Colafigli, M., De Luca, A., Fadda, G., Svicher, V., Alteri, C., Montano, M., D'Arrigo, R., Andreoni, M., Angarano, G., Antinori, A., Antonelli, G., Allice, T., Bagnarelli, P., Baldanti, F., Bertoli, A., Borderi, M., Boeri, E., ... Perno, C. F. (2012). Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group. New Microbiologica, 35, 17-25.

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title = "Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group",

abstract = "OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory. METHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004). RESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM. CONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.",

keywords = "Genotype, Genotyping Techniques, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Leukocytes, Mononuclear, Proviruses, Reproducibility of Results, Viral Load, Viral Tropism, Genotype, Genotyping Techniques, HIV Envelope Protein gp120, HIV Infections, HIV-1, Humans, Leukocytes, Mononuclear, Proviruses, Reproducibility of Results, Viral Load, Viral Tropism",

author = "Roberto Cauda and Rosaria Santangelo and Manuela Colafigli and {De Luca}, Andrea and Giovanni Fadda and Valentina Svicher and Claudia Alteri and Marco Montano and Roberta D'Arrigo and Massimo Andreoni and Gioacchino Angarano and Andrea Antinori and Guido Antonelli and Tiziano Allice and Patrizia Bagnarelli and Fausto Baldanti and Ada Bertoli and Marco Borderi and Enzo Boeri and Isabella Bon and Bianca Bruzzone and Callegaro, {Anna Paola} and Capobianchi, {Maria Rosaria} and Giampiero Carosi and Francesca Ceccherini-Silberstein and Massimo Clementi and Antonio Chirianni and {D'Arminio Monforte}, Antonella and {Di Biagio}, Antonio and {Di Nicuolo}, Giuseppe and {Di Perri}, Giovanni and {Di Pietro}, Massimo and {Di Santo}, Fabiola and Lavinia Fabeni and Massimo Galli and William Gennari and Valeria Ghisetti and Andrea Giacometti and Caterina Gori and Andrea Gori and Roberto Gulminetti and Francesco Leoncini and Gaetano Maffongelli and Franco Maggiolo and Giuseppe Manca and Franco Gargiulo and Canio Martinelli and Renato Maserati and Francesco Mazzotta and Genny Meini and Valeria Micheli and Laura Monno and Cristina Mussini and Pasquale Narciso and Silvia Nozza and Stefania Paolucci and Giorgio Pal{\`u} and Saverio Parisi and Giustino Parruti and Pignataro, {Angela Rosa} and Michela Pollicita and Tiziana Quirino and {Re Carla}, Maria and Giuliano Rizzardini and Renzo Scaggiante and Gaetana Sterrantino and Ombretta Turriziani and Vatteroni, {Maria Linda} and Laura Vecchi and Claudio Viscoli and Vincenzo Vullo and Maurizio Zazzi and Adriano Lazzarin and Perno, {Carlo Federico}",

year = "2012",

language = "English",

volume = "35",

pages = "17--25",

journal = "New Microbiologica",

issn = "1121-7138",

publisher = "-Pavia : Edizioni Internazionali, 2004 precedente: -Bologna: Luigi Ponzio e figlio",

}

Cauda, R , Santangelo, R, Colafigli, M, De Luca, A, Fadda, G, Svicher, V, Alteri, C, Montano, M, D'Arrigo, R, Andreoni, M, Angarano, G, Antinori, A, Antonelli, G, Allice, T, Bagnarelli, P, Baldanti, F, Bertoli, A, Borderi, M, Boeri, E, Bon, I, Bruzzone, B, Callegaro, AP, Capobianchi, MR, Carosi, G, Ceccherini-Silberstein, F, Clementi, M, Chirianni, A, D'Arminio Monforte, A, Di Biagio, A, Di Nicuolo, G, Di Perri, G, Di Pietro, M, Di Santo, F, Fabeni, L, Galli, M, Gennari, W, Ghisetti, V, Giacometti, A, Gori, C, Gori, A, Gulminetti, R, Leoncini, F, Maffongelli, G, Maggiolo, F, Manca, G, Gargiulo, F, Martinelli, C, Maserati, R, Mazzotta, F, Meini, G, Micheli, V, Monno, L, Mussini, C, Narciso, P, Nozza, S, Paolucci, S, Palù, G, Parisi, S, Parruti, G, Pignataro, AR, Pollicita, M, Quirino, T, Re Carla, M, Rizzardini, G, Scaggiante, R, Sterrantino, G, Turriziani, O, Vatteroni, ML, Vecchi, L, Viscoli, C, Vullo, V, Zazzi, M, Lazzarin, A & Perno, CF 2012, 'Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group', New Microbiologica, vol. 35, pagg. 17-25.

TY - JOUR

T1 - Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

AU - Cauda, Roberto

AU - Santangelo, Rosaria

AU - Colafigli, Manuela

AU - De Luca, Andrea

AU - Fadda, Giovanni

AU - Svicher, Valentina

AU - Alteri, Claudia

AU - Montano, Marco

AU - D'Arrigo, Roberta

AU - Andreoni, Massimo

AU - Angarano, Gioacchino

AU - Antinori, Andrea

AU - Antonelli, Guido

AU - Allice, Tiziano

AU - Bagnarelli, Patrizia

AU - Baldanti, Fausto

AU - Bertoli, Ada

AU - Borderi, Marco

AU - Boeri, Enzo

AU - Bon, Isabella

AU - Bruzzone, Bianca

AU - Callegaro, Anna Paola

AU - Capobianchi, Maria Rosaria

AU - Carosi, Giampiero

AU - Ceccherini-Silberstein, Francesca

AU - Clementi, Massimo

AU - Chirianni, Antonio

AU - D'Arminio Monforte, Antonella

AU - Di Biagio, Antonio

AU - Di Nicuolo, Giuseppe

AU - Di Perri, Giovanni

AU - Di Pietro, Massimo

AU - Di Santo, Fabiola

AU - Fabeni, Lavinia

AU - Galli, Massimo

AU - Gennari, William

AU - Ghisetti, Valeria

AU - Giacometti, Andrea

AU - Gori, Caterina

AU - Gori, Andrea

AU - Gulminetti, Roberto

AU - Leoncini, Francesco

AU - Maffongelli, Gaetano

AU - Maggiolo, Franco

AU - Manca, Giuseppe

AU - Gargiulo, Franco

AU - Martinelli, Canio

AU - Maserati, Renato

AU - Mazzotta, Francesco

AU - Meini, Genny

AU - Micheli, Valeria

AU - Monno, Laura

AU - Mussini, Cristina

AU - Narciso, Pasquale

AU - Nozza, Silvia

AU - Paolucci, Stefania

AU - Palù, Giorgio

AU - Parisi, Saverio

AU - Parruti, Giustino

AU - Pignataro, Angela Rosa

AU - Pollicita, Michela

AU - Quirino, Tiziana

AU - Re Carla, Maria

AU - Rizzardini, Giuliano

AU - Scaggiante, Renzo

AU - Sterrantino, Gaetana

AU - Turriziani, Ombretta

AU - Vatteroni, Maria Linda

AU - Vecchi, Laura

AU - Viscoli, Claudio

AU - Vullo, Vincenzo

AU - Zazzi, Maurizio

AU - Lazzarin, Adriano

AU - Perno, Carlo Federico

PY - 2012

Y1 - 2012

N2 - OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory. METHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004). RESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM. CONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

AB - OBJECTIVE: The DIVA study is aimed at setting up a standardized genotypic tropism-testing on proviral-DNA for the routine clinical diagnostic-laboratory. METHODS: Twelve local centres and 5 reference centres (previously cross-validated) were identified. For inter-center validation-procedure, 60 peripheral-blood mononuclear cells (PBMCs) aliquots from 45 HAART-treated patients were randomly chosen for population V3 sequencing on proviral-DNA at local HIV centre and at reference-laboratory. Viral tropism was predicted by Geno2Pheno algorithm (False Positive Rate [FPR] = 20%) as proposed by the European-Guidelines. Quantification of total HIV-1 DNA was based on a method described by Viard (2004). RESULTS: Quantification of HIV-1 DNA was available for 35/45 (77.8%) samples, and gave a median value of 598 (IQR:252- 1,203) copies/10 PBMCs. A total of 56/60 (93.3%) samples were successfully amplified by both the reference and the local virological centers. The overall concordance of tropism prediction between local and reference centers was 54/56 (96.4%). Results of tropism prediction by local centers were: 33/54 (61.1%) R5 and 21/54 (38.9%) X4/DM. CONCLUSION: There was high concordance in the genotypic tropism prediction based on proviral DNA among different virological centers throughout Italy. Our results are in line with other European studies, and support the use of genotypic tropism testing on proviral DNA in patients with suppressed plasma HIV-1 RNA candidate to CCR5-antagonist treatment.

KW - Genotype

KW - Genotyping Techniques

KW - HIV Envelope Protein gp120

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Leukocytes, Mononuclear

KW - Proviruses

KW - Reproducibility of Results

KW - Viral Load

KW - Viral Tropism

KW - Genotype

KW - Genotyping Techniques

KW - HIV Envelope Protein gp120

KW - HIV Infections

KW - HIV-1

KW - Humans

KW - Leukocytes, Mononuclear

KW - Proviruses

KW - Reproducibility of Results

KW - Viral Load

KW - Viral Tropism

UR - http://hdl.handle.net/10807/6319

M3 - Article

VL - 35

SP - 17

EP - 25

JO - New Microbiologica

JF - New Microbiologica

SN - 1121-7138

ER -

Performance of genotypic tropism testing on proviral DNA in clinical practice: results from the DIVA study group

Abstract

Keywords

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