Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS

Elisabetta Pisano, Tiziana Cabras, Rosanna Inzitari, Chiara Olmi, Massimo Castagnola, Irene Messana, Caterina Montaldo, Vincenzo Piras

Risultato della ricerca: Contributo in rivistaArticolo in rivista

71 Citazioni (Scopus)

Abstract

The acidic-soluble protein content of human gingival crevicular fluid was analyzed by reverse-phase high-performance liquid chromatography (RP-HPLC), and the eluent deriving from the chromatography separation was directly introduced into an ion-trap mass spectrometer through electrospray ionization (ESI-IT MS). By this technique the molecular weight of peptides/proteins was determined with a precision of approximate to 1/10,000 amu. On the basis of the chromatographic behavior and the knowledge of the molecular mass value, some peptides and proteins soluble in acidic solution were unambiguously recognized. Besides high quantities of human serum albumin, alpha-defensins 1-4 and minor amounts of cystatin A, statherin, basic PB salivary peptide and other unidentified components were detected. The presence of alpha-defensins in gingival crevicular fluid is in agreement with their relevant contribution to protein composition deriving from granulocyte secretions. Other peptides and proteins abundant in human saliva, such as proline-rich proteins (PRPs) and histatins, were not detected in gingival crevicular fluid. Further investigations will be necessary to establish the origin of statherin and PB salivary peptide in gingival crevicular fluid.
Lingua originaleEnglish
pagine (da-a)462-468
Numero di pagine7
RivistaEuropean Journal of Oral Sciences
Stato di pubblicazionePubblicato - 2005

Keywords

  • CREVICULAR FLUID
  • PEPTIDES
  • SALIVARY

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