Peptide fingerprinting of Hermetia illucens and Alphitobius diaperinus: Identification of insect species-specific marker peptides for authentication in food and feed

Giulia Leni, Stefano Sforza, Barbara Prandi, Martina Varani, Andrea Faccini, Augusta Caligiani

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Insects have been proposed as new source of proteins to meet the growing demand connected to the increasing world population. In the EU the inclusion of insect proteins in feed and food is strictly regulated. Hence, analytical methods able to discriminate and identify different insect species in food and feed are a necessity. In this work, a peptidomic approach was applied to determine peptide biomarkers for two edible insect species: lesser mealworm and black soldier fly. Three species specific peptide biomarkers were identified using LC-MS/MS. The two insects were mixed with fish standard feed at different concentrations, to evaluate the feasibility of their use as markers in complex matrices. The detection of marker peptides was confirmed down to 1% insect amount. The data here reported constitutes the first proof of concept for the potential application of the peptide marker approach for the identification and quantification of insect ingredients.
Lingua originaleEnglish
pagine (da-a)126681-126686
Numero di pagine6
RivistaFood Chemistry
DOI
Stato di pubblicazionePubblicato - 2020

Keywords

  • Authenticity
  • Feed
  • Insect
  • Peptide markers
  • Species Specificity
  • Biomarkers
  • Insect Proteins
  • Peptide Mapping
  • Animal Feed

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