Pentraxin 3 and complement cascade activation in the failure of arteriovenous fistula

Giuseppe Castellano, Angela Di Vittorio, Giuseppe Dalfino, Antonia Loverre, Daniela Marrone, Simona Simone, Francesco Paolo Schena, Giovanni Pertosa, Giuseppe Grandaliano

Risultato della ricerca: Contributo in rivistaArticolo in rivista


Objective: Pentraxin-3 (PTX3) has been suggested to play a role in the development of vascular pathology. Stenosis of arteriovenous fistula (AVF) leading to its failure is the major cause of morbidity in hemodialysis patients. To date, little is known on the pathogenesis of AVF stenosis. The aim of the present study was to investigate the potential role of PTX3 in this setting. Methods and results: A sample of venous wall was collected at the time of AVF formation in 44 patients with end stage renal disease. Ten patients developed AVF stenosis and from these patients a second portion of the venous wall was obtained during surgical revision of the AVF. Confocal laser scanning microscopy demonstrated that PTX3 immunostaining, hardly detectable in native AVF, was significantly increased in failed AVF, showing a specific co-localization with endothelial cell markers. Circulating mononuclear cells isolated at the time of AVF revision presented a significantly higher PTX3 mRNA expression than those collected during AVF creation. Interestingly, a significant deposition of C5b-9 on endothelial cells, co-localizing with PTX3, was observed in stenotic AVF. Conclusion: The present study demonstrates for the first time a close association between PTX3 deposition and complement activation at the endothelial cell level in failed AVF and suggests a role for PTX3 in modulating innate immunity in the pathogenesis of AVF stenosis. © 2009 Elsevier Ireland Ltd. All rights reserved.
Lingua originaleEnglish
pagine (da-a)241-247
Numero di pagine7
Stato di pubblicazionePubblicato - 2010


  • Adult
  • Aged
  • Arteriovenous Anastomosis
  • Arterovenous fistula
  • Biomarkers
  • C-Reactive Protein
  • Complement
  • Complement Activation
  • Complement Membrane Attack Complex
  • Constriction, Pathologic
  • Emodialysis
  • Endothelial cells
  • Endothelium, Vascular
  • Female
  • Humans
  • Kidney Failure, Chronic
  • Leukocytes, Mononuclear
  • Male
  • Middle Aged
  • Myocytes, Smooth Muscle
  • Pentraxin 3
  • RNA, Messenger
  • Renal Dialysis
  • Serum Amyloid P-Component
  • Treatment Failure


Entra nei temi di ricerca di 'Pentraxin 3 and complement cascade activation in the failure of arteriovenous fistula'. Insieme formano una fingerprint unica.

Cita questo