Pathogenic role of anti-beta 2 glycoprotein I antibodies in antiphospholipid associated fetal loss: characterisation of beta 2 glycoprotein I binding to trophoblast cells and functional effects of anti-beta 2 glycoprotein I antibodies in vitro

BACKGROUND: Antiphospholipid antibodies reacting with beta2-glycoprotein I (beta2GPI) have been associated with recurrent fetal loss and pregnancy complications. OBJECTIVE: To investigate whether specific mutations in the phospholipid binding site of beta2GPI might affect its binding to trophoblast and in turn the anti-beta2GPI antibody induced functional effects. METHODS: beta2GPI adhesion to trophoblast was evaluated as human monoclonal IgM or polyclonal IgG anti-beta2GPI antibody binding to trophoblast monolayers cultured (1) in complete medium; (2) in serum-free medium; (3) after serum starvation in the presence of purified human beta2GPI; or (4) in the presence of beta2GPI with single or multiple mutations in the amino acid loop Cys(281)-Lys-Asn-Lys-Glu-Lys-Lys-Cys(288). The effect of anti-beta2GPI binding to trophoblast was evaluated as chorionic gonadotropin (hCG) mRNA expression, and protein release by RT-PCR and radioimmunoassay, respectively. RESULTS: beta2GPI adhesion to trophoblast and its consequent recognition by the specific antibodies were inversely proportional to the mutation number in the phospholipid binding site. Anti-beta2GPI antibodies reduced gonadotropin release, hormone dependent hCG mRNA expression, and protein synthesis in the presence of beta2GPI, while the addition of the mutants or the absence of beta2GPI had no effect. CONCLUSIONS: beta2GPI binds to trophoblast in vitro through its fifth domain, as reported for endothelial cells, and can be recognised by anti-beta2GPI antibodies; the antibody binding downregulates trophoblast hCG synthesis and secretion. Such a mechanism might contribute to defective placentation in women with fetal loss associated with the antiphospholipid syndrome.