Paclitaxel directly binds to Bcl-2 and functionally mimics activity of Nur77

Giovanni Scambia, Cristiano Ferlini, Giuseppina Raspaglio, L Cicchillitti, S Bartollino, S Cimitan, C Bertucci, S Mozzetti, D Gallo, M Persico, C Fattorusso, G Campiani

Risultato della ricerca: Contributo in rivistaArticolo in rivista

104 Citazioni (Scopus)

Abstract

We reported previously that Bcl-2 is paradoxically down-regulated in paclitaxel-resistant cancer cells. We reveal here that paclitaxel directly targets Bcl-2 in the loop domain, thereby facilitating the initiation of apoptosis. Molecular modeling revealed an extraordinary similarity between the paclitaxel binding sites in Bcl-2 and beta-tubulin, leading us to speculate that paclitaxel could be mimetic of an endogenous peptide ligand, which binds both proteins. We tested the hypothesis that paclitaxel mimics Nur77, which, like paclitaxel, changes the function of Bcl-2. This premise was confirmed by Nur77 interacting with both paclitaxel targets (Bcl-2 and beta-tubulin) and a peptide sequence mimicking the Nur77 structural region, thus reproducing the paclitaxel-like effects of tubulin polymerization and opening the permeability transition pore channel in mitochondria. This discovery could help in the development of novel anticancer agents with nontaxane skeleton as well as in identifying the clinical subsets responsive to paclitaxel-based therapy.
Lingua originaleEnglish
pagine (da-a)6906-6914
Numero di pagine9
RivistaCancer Research
Volume69
DOI
Stato di pubblicazionePubblicato - 2009

Keywords

  • Animals
  • Antineoplastic Agents, Phytogenic
  • Apoptosis
  • Binding Sites
  • Cells, Cultured
  • DNA-Binding Proteins
  • Female
  • Humans
  • Mice
  • Mice, Nude
  • Mitochondria
  • Models, Molecular
  • Molecular Mimicry
  • Neoplasms
  • Nuclear Receptor Subfamily 4, Group A, Member 1
  • Paclitaxel
  • Peptide Fragments
  • Protein Binding
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Steroid
  • Tubulin
  • Tubulin Modulators

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