TY - JOUR
T1 - Oxidative stress in adult growth hormone deficiency: different plasma antioxidant patterns in comparison with metabolic syndrome
AU - Mancini, Antonio
AU - Di Segni, Chantal
AU - Bruno, Carmine
AU - Olivieri, Giulio
AU - Guidi, Francesco
AU - Silvestrini, Andrea
AU - Meucci Calabrese, Elisabetta
AU - Orlando, Patrick
AU - Silvestri, Sonia
AU - Tiano, Luca
AU - Pontecorvi, Alfredo
PY - 2018
Y1 - 2018
N2 - Background and aims: Growth hormone deficiency (GHD) is a condition associated with increased cardiovascular risk and insulin-resistance. Oxidative stress (OS) could be a mechanism underlying both these phenomena. In order to investigate plasma antioxidant defenses in such condition, we evaluated adults with GHD, compared with controls and metabolic syndrome patients (MetS), studying plasma total antioxidant capacity (TAC) and coenzyme Q10 (CoQ10, lipophilic antioxidant) levels, both in its oxidized and reduced forms, correlating this data with metabolic and hormonal pattern. Materials and methods: In this case-control study, 51 GHD, 36 controls, and 35 MetS were enrolled. An evaluation of hormonal and metabolic parameters was performed. TAC was measured using the system metmyoglobin -H202and the chromogen ABTS, whose radical form is spectroscopically revealed; latency time (LAG) in the appearance of ABTS●is proportional to antioxidant in sample. CoQ10 was assayed by electrochemical method. Results: Despite HOMA index was higher in both GHD and MetS (2.2 ± 0.3 and 3.1 ± 0.3 vs. 1.2 ± 0.2 in controls), only in MetS we observed lower LAG levels (64.5 ± 3.1 s vs. 82.8 ± 5.8 in GHD and 80.6 ± 6.6 in controls), suggesting an increased consumption of antioxidants. LAG significantly correlated with uric acid only in MetS (r2= 0.65, p < 0.001), suggesting a different pattern of antioxidants. CoQ10 exhibited a trend toward lower levels in GHD, although not significant. Conclusions: Our data indicate that GHD, although sharing with MetS various metabolic features, including increased HOMA levels, showed a different pattern of plasma antioxidants, suggesting inadequate reactivity toward radical production rather than an antioxidants consumption as in MetS.
AB - Background and aims: Growth hormone deficiency (GHD) is a condition associated with increased cardiovascular risk and insulin-resistance. Oxidative stress (OS) could be a mechanism underlying both these phenomena. In order to investigate plasma antioxidant defenses in such condition, we evaluated adults with GHD, compared with controls and metabolic syndrome patients (MetS), studying plasma total antioxidant capacity (TAC) and coenzyme Q10 (CoQ10, lipophilic antioxidant) levels, both in its oxidized and reduced forms, correlating this data with metabolic and hormonal pattern. Materials and methods: In this case-control study, 51 GHD, 36 controls, and 35 MetS were enrolled. An evaluation of hormonal and metabolic parameters was performed. TAC was measured using the system metmyoglobin -H202and the chromogen ABTS, whose radical form is spectroscopically revealed; latency time (LAG) in the appearance of ABTS●is proportional to antioxidant in sample. CoQ10 was assayed by electrochemical method. Results: Despite HOMA index was higher in both GHD and MetS (2.2 ± 0.3 and 3.1 ± 0.3 vs. 1.2 ± 0.2 in controls), only in MetS we observed lower LAG levels (64.5 ± 3.1 s vs. 82.8 ± 5.8 in GHD and 80.6 ± 6.6 in controls), suggesting an increased consumption of antioxidants. LAG significantly correlated with uric acid only in MetS (r2= 0.65, p < 0.001), suggesting a different pattern of antioxidants. CoQ10 exhibited a trend toward lower levels in GHD, although not significant. Conclusions: Our data indicate that GHD, although sharing with MetS various metabolic features, including increased HOMA levels, showed a different pattern of plasma antioxidants, suggesting inadequate reactivity toward radical production rather than an antioxidants consumption as in MetS.
KW - Antioxidants
KW - Coenzyme Q10
KW - Endocrinology
KW - Endocrinology, Diabetes and Metabolism
KW - Insulin-resistance
KW - Metabolic syndrome
KW - Pituitary
KW - Precision medicine
KW - Antioxidants
KW - Coenzyme Q10
KW - Endocrinology
KW - Endocrinology, Diabetes and Metabolism
KW - Insulin-resistance
KW - Metabolic syndrome
KW - Pituitary
KW - Precision medicine
UR - http://hdl.handle.net/10807/120571
UR - http://www.springer.com/humana+press/journal/12020
U2 - 10.1007/s12020-017-1468-1
DO - 10.1007/s12020-017-1468-1
M3 - Article
SN - 1355-008X
VL - 59
SP - 130
EP - 136
JO - Endocrine
JF - Endocrine
ER -