TY - CHAP
T1 - Overexpression of PRV-1 gene in polycythemia rubra vera and essential thrombocythemia
AU - Martini, Maurizio
AU - Teofili, Luciana
AU - Larocca, Luigi Maria
PY - 2006
Y1 - 2006
N2 - The polycythemia rubra vera 1 gene (PRV-1), a member of the urokinase-type plasminogen activator receptor superfamily, is overexpressed in granulocytes isolated from the peripheral blood of patients with polycythemia vera (PV) and essential thrombocythemia (ET). PRV-1 overexpression is the first reliable molecular marker of these myeloproliferative disorders, and its detection allows us to discriminate PV and ET from secondary erythrocytosis and thrombocytosis. PRV-1 overexpression can be investigated by several techniques, including Northern analysis, reverse-transcription (RT)-polymerase chain reaction (PCR), and real-time PCR. Among these, RT-PCR is the most rapid, reliable, and feasible method for the detection of PRV-1 overexpression in highly purified peripheral blood granulocytes.
AB - The polycythemia rubra vera 1 gene (PRV-1), a member of the urokinase-type plasminogen activator receptor superfamily, is overexpressed in granulocytes isolated from the peripheral blood of patients with polycythemia vera (PV) and essential thrombocythemia (ET). PRV-1 overexpression is the first reliable molecular marker of these myeloproliferative disorders, and its detection allows us to discriminate PV and ET from secondary erythrocytosis and thrombocytosis. PRV-1 overexpression can be investigated by several techniques, including Northern analysis, reverse-transcription (RT)-polymerase chain reaction (PCR), and real-time PCR. Among these, RT-PCR is the most rapid, reliable, and feasible method for the detection of PRV-1 overexpression in highly purified peripheral blood granulocytes.
KW - Diagnosis, Differential
KW - GPI-Linked Proteins
KW - Gene Expression Regulation
KW - Granulocytes
KW - Humans
KW - Isoantigens
KW - Membrane Glycoproteins
KW - Polycythemia Vera
KW - RNA
KW - RNA, Messenger
KW - Receptors, Cell Surface
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Thrombocythemia, Essential
KW - Diagnosis, Differential
KW - GPI-Linked Proteins
KW - Gene Expression Regulation
KW - Granulocytes
KW - Humans
KW - Isoantigens
KW - Membrane Glycoproteins
KW - Polycythemia Vera
KW - RNA
KW - RNA, Messenger
KW - Receptors, Cell Surface
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Thrombocythemia, Essential
UR - http://hdl.handle.net/10807/25961
M3 - Chapter
SN - NA
SP - 265
EP - 273
BT - Methods in Molecular Medicine
ER -