Ovarian senescence increases liver fibrosis in humans and zebrafish with steatosis

Luca Miele, Antonio Grieco, Elena Turola, Salvatore Petta, Ester Vanni, Fabiola Milosa, Luca Valenti, Rosina Critelli, Livia Maccio, Vincenza Calvaruso, Anna L. Fracanzani, Marcello Bianchini, Nazarena Raos, Elisabetta Bugianesi, Serena Mercorella, Marisa Di Giovanni, Antonio Craxì, Silvia Fargion, Calogero Cammà, Franco CotelliErica Villa

Risultato della ricerca: Contributo in rivistaArticolo in rivista

28 Citazioni (Scopus)

Abstract

Contrasting data exist on the effect of gender and menopause on the susceptibility, development and liver damage progression in non-alcoholic fatty liver disease (NAFLD). Our aim was to assess whether menopause is associated with the severity of liver fibrosis in individuals with NAFLD and to explore the issue of ovarian senescence in experimental liver steatosis in zebrafish. In 244 females and age-matched males with biopsy-proven NAFLD, we assessed anthropometric, biochemical and metabolic features, including menopausal status (self-reported); liver biopsy was scored according to 'The Pathology Committee of the NASH Clinical Research Network'. Young and old male and female zebrafish were fed for 24 weeks with a high-calorie diet. Weekly body mass index (BMI), histopathological examination and quantitative real-time PCR analysis on genes involved in lipid metabolism, inflammation and fibrosis were performed. In the entire cohort, at multivariate logistic regression, male gender [odds ratio (OR): 1.408, 95% confidence interval (95% CI): 0.779-2.542, P=0.25] vs women at reproductive age was not associated with F2-F4 fibrosis, whereas a trend was observed for menopause (OR: 1.752, 95% CI: 0.956-3.208, P=0.06). In women, menopause (OR: 2.717, 95% CI: 1.020-7.237, P=0.04) was independently associated with F2-F4 fibrosis. Similarly, in overfed zebrafish, old female fish with failing ovarian function [as demonstrated by extremely low circulating estradiol levels (1.4±0.1 pg/µl) and prevailing presence of atretic follicles in the ovaries] developed massive steatosis and substantial fibrosis (comparable with that occurring in males), whereas young female fish developed less steatosis and were totally protected from the development of fibrosis. Ovarian senescence significantly increases the risk of fibrosis severity both in humans with NAFLD and in zebrafish with experimental steatosis.
Lingua originaleEnglish
pagine (da-a)1037-1046
Numero di pagine10
RivistaDISEASE MODELS & MECHANISMS
Volume8
DOI
Stato di pubblicazionePubblicato - 2015

Keywords

  • Fibrosis
  • Menopause
  • Non-alcoholic fatty liver disease
  • Ovarian senescence
  • Zebrafish

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