TY - JOUR
T1 - Optical discrimination of pathological red blood cells
AU - Vergari, Michele
AU - Niccolini, Benedetta
AU - Pitocco, Dario
AU - Rizzi, Alessandro
AU - Ciasca, Gabriele
AU - De Spirito, Marco
AU - Gavioli, Luca
PY - 2024
Y1 - 2024
N2 - Fast diagnostic methods are crucial to reduce the burden on healthcare systems. Currently, detection of diabetes complications such as neuropathy requires time-consuming approaches to observe the correlated red blood cells (RBCs) morphological changes. To tackle this issue, an optical analysis of RBCs in air was conducted in the 250-2500 nm range. The distinct oscillations present in the scattered and direct transmittance spectra have been analyzed with both Mie theory and anomalous diffraction approximation. The results provide information about the swelling at the ends of RBCs and directly relate the optical data to RBCs morphology and deformability. Both models agree on a reduction in the size and deformability of RBCs in diabetic patients, thus opening the way to diabetes diagnosis and disease progression assessment.
AB - Fast diagnostic methods are crucial to reduce the burden on healthcare systems. Currently, detection of diabetes complications such as neuropathy requires time-consuming approaches to observe the correlated red blood cells (RBCs) morphological changes. To tackle this issue, an optical analysis of RBCs in air was conducted in the 250-2500 nm range. The distinct oscillations present in the scattered and direct transmittance spectra have been analyzed with both Mie theory and anomalous diffraction approximation. The results provide information about the swelling at the ends of RBCs and directly relate the optical data to RBCs morphology and deformability. Both models agree on a reduction in the size and deformability of RBCs in diabetic patients, thus opening the way to diabetes diagnosis and disease progression assessment.
KW - Mie theory
KW - anomalous diffraction approximation
KW - erythrocyte light scattering
KW - erythrocyte morphology
KW - optical spectrum correlation with patient's health conditions
KW - optical transmission and reflection
KW - Mie theory
KW - anomalous diffraction approximation
KW - erythrocyte light scattering
KW - erythrocyte morphology
KW - optical spectrum correlation with patient's health conditions
KW - optical transmission and reflection
UR - http://hdl.handle.net/10807/294576
U2 - 10.1002/bit.28798
DO - 10.1002/bit.28798
M3 - Article
SN - 0006-3592
VL - 121
SP - 3311
EP - 3318
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
ER -