TY - JOUR
T1 - On-line Solid Phase Extraction coupled to Liquid Chromatography Ion Trap Tandem Mass Spectrometry for Determination of Penicillins in Catfish
AU - Maggi, Luana
AU - De Mendoza, Jorge Hurtado
AU - Zalacain, Amaya
AU - Bonetto, Liliana
AU - Mocholí, Francisco A.
AU - Carmona, Manuel
PY - 2012
Y1 - 2012
N2 - A highly selective method was developed for the simultaneous determination of eight penicillins in catfish using automated on-line solid-phase extraction coupled to liquid chromatography–tandem mass spectrometry
(XLC–MS/MS). The type of cartridge, equilibration sample volume, volume of solvent to carry the sample into the cartridge, and elution times were studied in order to optimize the XLC operating conditions. MS/MS conditions
were also adjusted for better peak resolution. The present method was validated in agreement with the criteria of Commission Decision 2002/657/EC, showing a linear range from 2 to 350 μg kg−1 and regression coefficient higher than 0.995 for the studied penicillins. Decision limits, calculated in the case of substances with no permitted limit, were lower than 0.6 μg kg−1, and detection capability values were lower than 2.0 μg kg−1. Samples spiked at 2.0, 10.0, and 50.0 μg kg−1 showed high recovery (72–92%) and precision values lower than 20% except for amoxicillin. The
present method was also applied for the analysis of penicillins in 30 catfish samples bought in local markets.
AB - A highly selective method was developed for the simultaneous determination of eight penicillins in catfish using automated on-line solid-phase extraction coupled to liquid chromatography–tandem mass spectrometry
(XLC–MS/MS). The type of cartridge, equilibration sample volume, volume of solvent to carry the sample into the cartridge, and elution times were studied in order to optimize the XLC operating conditions. MS/MS conditions
were also adjusted for better peak resolution. The present method was validated in agreement with the criteria of Commission Decision 2002/657/EC, showing a linear range from 2 to 350 μg kg−1 and regression coefficient higher than 0.995 for the studied penicillins. Decision limits, calculated in the case of substances with no permitted limit, were lower than 0.6 μg kg−1, and detection capability values were lower than 2.0 μg kg−1. Samples spiked at 2.0, 10.0, and 50.0 μg kg−1 showed high recovery (72–92%) and precision values lower than 20% except for amoxicillin. The
present method was also applied for the analysis of penicillins in 30 catfish samples bought in local markets.
KW - Antibiotics
KW - Catfish
KW - On-line solid-phase extraction
KW - Penicillins
KW - XLC–MS/MS
KW - Antibiotics
KW - Catfish
KW - On-line solid-phase extraction
KW - Penicillins
KW - XLC–MS/MS
UR - http://hdl.handle.net/10807/29374
U2 - 10.1007/s12161-011-9342-x
DO - 10.1007/s12161-011-9342-x
M3 - Article
SN - 1936-9751
VL - 5
SP - 1047
EP - 1053
JO - Food Analytical Methods
JF - Food Analytical Methods
ER -