TY - JOUR
T1 - Oligosaccharides in 4 different milk groups, Bifidobacteria, and Ruminococcus obeum.
AU - Coppa, Giovanni V.
AU - Gabrielli, Orazio
AU - Zampini, Lucia
AU - Galeazzi, Tiziana
AU - Ficcadenti, Anna
AU - Padella, Lucia
AU - Santoro, Lucia
AU - Soldi, Sara
AU - Carlucci, Antonio
AU - Bertino, Enrico
AU - Morelli, Lorenzo
PY - 2011
Y1 - 2011
N2 - Objectives: The aim was of this study is to identify a link between the total
amount of breast milk oligosaccharides and faecal microbiota composition
of newborns at the end of the first month of life, with special attention paid to
bifidobacteria, and establish the role, if any, of the different oligosaccharides
in determining the gut microbiota composition.
Subjects and Methods: Milk oligosaccharide groups were identified by
high-performance anion exchange chromatography analysis. DPCRNA
from newborns’ faecal samples at 30 days of life was isolated and
processed by polymerase chain reaction analyses that allow the
identification of 6 species of bifidobacteria (adolescentis, bifidum, breve,
catenulatum, longum, infantis) and Ruminococcus spp; denaturing gradient
gel electrophoresis analysis was also performed.
Results: No substantial differences in bifidobacteria species composition
within milk groups 1, 2, and 3 were observed; however, infants fed with
group 4 milk show a microbiota characterised by a greater frequency of
Bifidobacteria adolescentis and the absence of Bifidobacteria catenulatum.
For the first time, a high percentage of the Ruminococcus genus in infants fed
with all milk groups was found.
Conclusions: Our data show that milk groups 1, 2, and 3, containing an
amount of oligosaccharides ranging within 10 to 15 g/L, share a substantially
identical composition of the intestinal microbiota in breast-fed infants,
despite quali-quantitative difference in oligosaccharides content.
Newborns taking milk with only 5 g/L of oligosaccharides (group 4)
harbour a different intestinal microbiota.
AB - Objectives: The aim was of this study is to identify a link between the total
amount of breast milk oligosaccharides and faecal microbiota composition
of newborns at the end of the first month of life, with special attention paid to
bifidobacteria, and establish the role, if any, of the different oligosaccharides
in determining the gut microbiota composition.
Subjects and Methods: Milk oligosaccharide groups were identified by
high-performance anion exchange chromatography analysis. DPCRNA
from newborns’ faecal samples at 30 days of life was isolated and
processed by polymerase chain reaction analyses that allow the
identification of 6 species of bifidobacteria (adolescentis, bifidum, breve,
catenulatum, longum, infantis) and Ruminococcus spp; denaturing gradient
gel electrophoresis analysis was also performed.
Results: No substantial differences in bifidobacteria species composition
within milk groups 1, 2, and 3 were observed; however, infants fed with
group 4 milk show a microbiota characterised by a greater frequency of
Bifidobacteria adolescentis and the absence of Bifidobacteria catenulatum.
For the first time, a high percentage of the Ruminococcus genus in infants fed
with all milk groups was found.
Conclusions: Our data show that milk groups 1, 2, and 3, containing an
amount of oligosaccharides ranging within 10 to 15 g/L, share a substantially
identical composition of the intestinal microbiota in breast-fed infants,
despite quali-quantitative difference in oligosaccharides content.
Newborns taking milk with only 5 g/L of oligosaccharides (group 4)
harbour a different intestinal microbiota.
KW - Bifidobacterium
KW - breast-fed infants
KW - human milk
KW - Bifidobacterium
KW - breast-fed infants
KW - human milk
UR - http://hdl.handle.net/10807/7493
M3 - Article
SN - 0277-2116
VL - 2011
SP - 80
EP - 87
JO - Journal of Pediatric Gastroenterology and Nutrition
JF - Journal of Pediatric Gastroenterology and Nutrition
ER -