Ochratoxin a removal by Lactobacillus plantarum V22 in synthetic substrates

Roberta Dordoni, Giorgia Spigno, Alessandro Moncalvo, Angela Silva, Maria Daria Fumi, S. Di Piazza

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Background: Ochratoxin A is a nephrotoxin which may occur in wines characterised by higher pH than the average. In the last decades the mechanisms responsible for ochratoxin A reduction by lactic acid bacteria have been investigated and identified as mainly cell walls adsorption and / or enzymatic conversion to ochratoxin-α, a non-toxic metabolite. Since lactic acid bacteria are involved in the malolactic fermentation during the wine-making process, selected starter cultures could be exploited to guarantee safe ochratoxin A level in wines also from contaminated grapes. A lactic acid bacteria strain (Lactobacillus plantarum V22) was previously selected for its ability of both degrading ochratoxin A and carrying out malolactic fermentation at high pH. Objective: This study was aimed at assessing if the selected L. plantarum strain, can reduce ochratoxin A because it can use it as a carbon source. Methods: L. plantarum V22 was grown in the presence of ochratoxin A in two different synthetic substrates, with or without malic acid, monitoring the reduction of ochratoxin A and the presence of ochratoxin α as an indicator for a toxin enzymatic hydrolysis. The presence of residual not hydrolysed ochratoxin A bound to the bacteria cell walls was also evaluated to quantify the ochratoxin A removal due to simple adsorption. Result: A significant reduction of 19.5 ± 2.0% in ochratoxin A concentration was observed only in the presence of malic acid. The quantified fraction of ochratoxin A adsorbed on cell walls was irrelevant and the metabolite ochratoxin α could not be detected. Conclusion: There is a possibility that L. plantarum V22 can degrade ochratoxin A through a not yet identified metabolic pathway.
Lingua originaleEnglish
pagine (da-a)282-287
Numero di pagine6
RivistaTHE OPEN BIOTECHNOLOGY JOURNAL
Volume12
DOI
Stato di pubblicazionePubblicato - 2018

Keywords

  • Bio-decontamination
  • Biochemistry
  • Bioengineering
  • Biomedical Engineering
  • Biophysics
  • Biotechnology
  • Lactobacillus plantarum
  • Malolactic fermentation
  • Ochratoxin A
  • Synthetic Substrates
  • Wine

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