TY - JOUR
T1 - Nuclear magnetic resonance-based metabolomics of exhaled breath condensate: methodological aspects
AU - Motta, A.
AU - Paris, D.
AU - Melck, D.
AU - De Laurentiis, G.
AU - Maniscalco, M.
AU - Sofia, M.
AU - Montuschi, Paolo
PY - 2012
Y1 - 2012
N2 - The lack of standardized procedures for exhaled breath condensate (EBC), a noninvasive technique for investigating lung inflammatory mediators, makes it difficult between-laboratory comparison of results. Moreover, different collecting devices have been reported to influence the EBC content.
Analysis of metabolic profiles (“metabolomics”) of EBC with nuclear magnetic resonance (NMR) spectroscopy discriminates between chronic obstructive pulmonary disease (COPD) patients and healthy subjects (HS), between asthmatic children and HS, and between patients with stable cystic fibrosis, unstable cystic fibrosis and HS.
This approach has recently been questioned, as NMR-based metabolomics of EBC collected using a condenser with reusable parts was reported to be affected by cleaning procedures, generating artificial signals not related to the endogenous metabolites of the lungs.
In this study, we assessed: the effects of a different cleaning procedure of a reusable-part condenser on EBC metabolomics; the possible time and carry-over effects when the same device is repeatedly used; technique sensitivity; the ability of NMR spectroscopy of EBC to discriminate between COPD patients and HS; its potential for identifying selective EBC metabolites.
AB - The lack of standardized procedures for exhaled breath condensate (EBC), a noninvasive technique for investigating lung inflammatory mediators, makes it difficult between-laboratory comparison of results. Moreover, different collecting devices have been reported to influence the EBC content.
Analysis of metabolic profiles (“metabolomics”) of EBC with nuclear magnetic resonance (NMR) spectroscopy discriminates between chronic obstructive pulmonary disease (COPD) patients and healthy subjects (HS), between asthmatic children and HS, and between patients with stable cystic fibrosis, unstable cystic fibrosis and HS.
This approach has recently been questioned, as NMR-based metabolomics of EBC collected using a condenser with reusable parts was reported to be affected by cleaning procedures, generating artificial signals not related to the endogenous metabolites of the lungs.
In this study, we assessed: the effects of a different cleaning procedure of a reusable-part condenser on EBC metabolomics; the possible time and carry-over effects when the same device is repeatedly used; technique sensitivity; the ability of NMR spectroscopy of EBC to discriminate between COPD patients and HS; its potential for identifying selective EBC metabolites.
KW - Exhaled breath condensate
KW - Metabolomics
KW - NMR spectroscopy
KW - Exhaled breath condensate
KW - Metabolomics
KW - NMR spectroscopy
UR - http://hdl.handle.net/10807/4235
U2 - 10.1183/09031936.00036411
DO - 10.1183/09031936.00036411
M3 - Article
SN - 1399-3003
VL - 39
SP - 498
EP - 500
JO - European Respiratory Journal
JF - European Respiratory Journal
ER -