Novel sensitive, specific and rapid pharmacogenomic test for the prediction of abacavir hypersensitivity reaction: HLA-B*57:01 detection by real-time PCR.

Cinzia Dello Russo, Lucia Lisi, Simona Di Giambenedetto, Roberto Cauda, Pierluigi Navarra, Bruno Federico, Andrea De Luca, 33189, DI MEDICINA E CHIRURGIA "A.GEMELLI" FACOLTA', ROMA - Dipartimento di Sicurezza e Bioetica, Alessia Lofaro, Giordano Madeddu, Marianna Salerno, Maria Stella Mura, Antonella Pirazzoli

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

28 Citazioni (Scopus)

Abstract

Aim: International HIV treatment guidelines recommend HLA-B*57:01 typing before abacavir administration, in order to reduce the incidence of abacavir hypersensitivity reactions, the major cause of early therapy discontinuation. A fast, sensitive and specific test for HLA-B*57:01 detection has been developed in the present study. Materials & methods: Two sets of sequence-specific primers were designed, and amplification rapidly detected by real-time PCR. Results: A total of 108 samples were analyzed in a single-blind fashion, and 41 samples were identified as positive. Complete agreement, with k = 1 (standard error = 0.0962, p < 0.0001), was found, with a validated methodology used in the EPI109367 clinical trial funded by GlaxoSmithKline, and consisting of low-resolution sequence-specific oligonucleotide PCR, followed by high-resolution sequence-specific oligonucleotide PCR carried out on the HLA-B*57-positive samples. Conclusion: We provided a detailed characterization of a novel HLA-B*57:01 screening test, which can be easily implemented by those laboratories already involved in the detection of viral load and virus genotyping.
Lingua originaleEnglish
pagine (da-a)567-576
Numero di pagine10
RivistaPharmacogenomics
Volume12
Stato di pubblicazionePubblicato - 2011

Keywords

  • HLA-B*57:01
  • Hypersensitivity
  • SSP
  • abacavir
  • real-time PCR
  • screening

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