Novel sensitive, specific and rapid pharmacogenomic test for the prediction of abacavir hypersensitivity reaction: HLA-B*57:01 detection by real-time PCR.

Cinzia Dello Russo, Lucia Lisi, Simona Di Giambenedetto, Roberto Cauda, Pierluigi Navarra, Bruno Federico, Andrea De Luca, Alessia Lofaro, Giordano Madeddu, Marianna Salerno, Maria Stella Mura, Antonella Pirazzoli

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

28 Citazioni (Scopus)

Abstract

Aim: International HIV treatment guidelines recommend HLA-B*57:01 typing before abacavir administration, in order to reduce the incidence of abacavir hypersensitivity reactions, the major cause of early therapy discontinuation. A fast, sensitive and specific test for HLA-B*57:01 detection has been developed in the present study. Materials & methods: Two sets of sequence-specific primers were designed, and amplification rapidly detected by real-time PCR. Results: A total of 108 samples were analyzed in a single-blind fashion, and 41 samples were identified as positive. Complete agreement, with k = 1 (standard error = 0.0962, p < 0.0001), was found, with a validated methodology used in the EPI109367 clinical trial funded by GlaxoSmithKline, and consisting of low-resolution sequence-specific oligonucleotide PCR, followed by high-resolution sequence-specific oligonucleotide PCR carried out on the HLA-B*57-positive samples. Conclusion: We provided a detailed characterization of a novel HLA-B*57:01 screening test, which can be easily implemented by those laboratories already involved in the detection of viral load and virus genotyping.
Lingua originaleEnglish
pagine (da-a)567-576
Numero di pagine10
RivistaPharmacogenomics
Volume12
Stato di pubblicazionePubblicato - 2011

Keywords

  • HLA-B*57:01
  • Hypersensitivity
  • SSP
  • abacavir
  • real-time PCR
  • screening

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