TY - JOUR
T1 - NOVEL BIOMARKERS OF ANDROGEN DEFICIENCY FROM SEMINAL PLASMA PROFILING USING HIGH-RESOLUTION MASS SPECTROMETRY.
AU - Milardi, Domenico
AU - Grande, Giuseppe
AU - Vincenzoni, Federica
AU - Giampietro, Antonella
AU - Messana, Irene
AU - Castagnola, Massimo
AU - Marana, Riccardo
AU - De Marinis, Laura
AU - Pontecorvi, Alfredo
PY - 2014
Y1 - 2014
N2 - CONTEXT: The seminal plasma is made out of secretions from the testis, the epididymis and the male accessory glands, that are dependent upon the presence of androgenic stimuli. OBJECTIVE: The objective of this study was to identify new seminal biomarkers for secundary male hypogonadism using proteomic profiling. DESIGN: Seminal plasma samples from patients affected by secundary hypogonadism and normogonadal controls were analyzed by an LTQ Orbitrap XL hybrid mass spectrometer and\r\ndata were evaluated using bioformatic tools. SETTING: The study was performed at a clinical research center. SUBJECTS: Twenty male patients, aged 25-55 yr, affected by secundary hypogonadic were studied. Ten patients were re-evaluated after 6 months of testosterone replacement therapy(TRT). Ten normogonadic men were enrolled as control group.INTERVENTIONS: none\r\nMAIN OUTCOME MEASURES: The list of absent proteins in the samples of hypogonadic patients and identified after TRT was studied. Bioinformatic tools were used to functionally annotate the panel of androgen-dependent proteins. The interaction network of the differentially expressed proteins was built in silico, including the androgen receptor (AR). RESULTS: A lower number of proteins was identified in hypogonadic patients compared with normogonadal men. Among the 61 proteins identified in normogonadal men, proteins\r\n44 were absent in hypogonadic patients. 14 out 33 absent proteins were identified in seminal samples after 6 months of TRT. Functional annotation analysis revealed that binding and\r\n46 enzymatic activities are mainly deficient in male hypogonadism. 7 out of 14 differentially expressed proteins can fall into one large protein-protein interaction network, which directly\r\n48 involves the AR.
AB - CONTEXT: The seminal plasma is made out of secretions from the testis, the epididymis and the male accessory glands, that are dependent upon the presence of androgenic stimuli. OBJECTIVE: The objective of this study was to identify new seminal biomarkers for secundary male hypogonadism using proteomic profiling. DESIGN: Seminal plasma samples from patients affected by secundary hypogonadism and normogonadal controls were analyzed by an LTQ Orbitrap XL hybrid mass spectrometer and\r\ndata were evaluated using bioformatic tools. SETTING: The study was performed at a clinical research center. SUBJECTS: Twenty male patients, aged 25-55 yr, affected by secundary hypogonadic were studied. Ten patients were re-evaluated after 6 months of testosterone replacement therapy(TRT). Ten normogonadic men were enrolled as control group.INTERVENTIONS: none\r\nMAIN OUTCOME MEASURES: The list of absent proteins in the samples of hypogonadic patients and identified after TRT was studied. Bioinformatic tools were used to functionally annotate the panel of androgen-dependent proteins. The interaction network of the differentially expressed proteins was built in silico, including the androgen receptor (AR). RESULTS: A lower number of proteins was identified in hypogonadic patients compared with normogonadal men. Among the 61 proteins identified in normogonadal men, proteins\r\n44 were absent in hypogonadic patients. 14 out 33 absent proteins were identified in seminal samples after 6 months of TRT. Functional annotation analysis revealed that binding and\r\n46 enzymatic activities are mainly deficient in male hypogonadism. 7 out of 14 differentially expressed proteins can fall into one large protein-protein interaction network, which directly\r\n48 involves the AR.
KW - ANDROGEN DEFICIENCY
KW - ANDROGEN DEFICIENCY
UR - https://publicatt.unicatt.it/handle/10807/57533
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84905860797&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84905860797&origin=inward
U2 - 10.1210/jc.2013-4148
DO - 10.1210/jc.2013-4148
M3 - Article
SN - 0021-972X
VL - 99
SP - 2813
EP - 2820
JO - Journal of Clinical Endocrinology and Metabolism
JF - Journal of Clinical Endocrinology and Metabolism
IS - 8
ER -