Nicotine regulates basic fibroblastic growth factor and transforming growth factor beta1 production in endothelial cells.

Valentina Corvino, A Cucina, P Sapienza, V Borrelli, M Lucarelli, S Scarpa, R Strom, D'Angelo L Santoro, A. Cavallaro

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

Nicotine, a constituent of cigarette smoking, may induce atherosclerosis through the production of growth factors. The pattern of bFGF and TGF b1 production and release by bovine aortic endothelial cells (EC) stimulated with nicotine (from 6 3 1024 to 6 3 1028 M) was studied. EC viability and count were assessed. The presence of bFGF and TGF b1 in serum-free conditioned media was determined by the inhibition antibody-binding assay and Western blot analysis. Mitogenic activity of nicotine on EC was also determined. Polymerase chain reaction (PCR) was used to study the expression of bFGF and TGF b1. The bFGF release after nicotine stimulation was greater than controls, whereas TGF b1 release was lower. At a nicotine concentration of 6 3 1026 Mwe noted the greatest mitogenic activity. The addition of monoclonal antibody anti-bFGF decreased the tritiated thymidine uptake of EC exposed to nicotine but the addition of monoclonal antibody anti-TGF b1 had no significant effect. bFGF mRNA expression was significantly higher in EC exposed to nicotine than in controls, whereas TGF b1 mRNA expression was not modified. From these data we concluded that nicotine regulates bFGF production and release and TGF b1 release and may have a key role in the development and progression of atherosclerosis
Lingua originaleEnglish
pagine (da-a)306-312
Numero di pagine7
RivistaBiochemical and Biophysical Research Communications
Stato di pubblicazionePubblicato - 1999

Keywords

  • Nicotine
  • TGFbeta
  • aortic endothelial cells
  • bFGF

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