Several substances, once injected in a given central structure, are taken up by axon terminals and transported
retrogradely over long distances, to the cell bodies of neurons projecting to the injection area. They are
then visualized by means of histochemical (or immunohistochemical) reactions, making it possible to trace
neural pathways .
Here we describe a method to label retrogradely neocortical pyramidal neurons in a Golgi-like fashion.
This method allows reconstructing the entire dendritic tree of retrogradely labeled cells and can be easily combined with immunohistochemical techniques. Methods suitable to establish relationships
between morphological and functional features of projecting neurons are also discussed.
|Titolo della pubblicazione ospite||Immunocytochemistry and Related Techniques|
|Editor||Adalberto Merighi, Laura Lossi|
|Numero di pagine||16|
|Stato di pubblicazione||Pubblicato - 2015|
- Pyramidal neurons