Neuroglobin-prion protein interaction: what's the function?

Alessandro Arcovito, Giovanni Luca Scaglione, Pasquale Palladino, Rosa Maria Vitale, Pietro Amodeo, Beatrice Vallone, Maurizio Brunori, Ettore Benedetti, Filomena Rossi

Risultato della ricerca: Contributo in rivistaArticolo in rivista

13 Citazioni (Scopus)

Abstract

Neuroglobin and cellular prion protein (PrPC) are expressed in the nervous system and co-localized in the retinal ganglion cell layer. Both proteins do not have an unambiguously assigned function, and it was recently reported that PrPC aggregates rapidly in the presence of neuroglobin, whereas it does not aggregate in the presence of myoglobin, another globin with different tissue specificity. Electrostatic complementarity between the unstructured PrPCN-terminus and neuroglobin has been proposed to mediate this specific interaction. To verifythis hypothesis experimentally, we have used a combined approach of automated docking and molecular dynamics (MD) studies carried out on short stretches of prion protein (PrP) N-terminus to identify the minimal electrostatically interacting aminoacidic sequences with neuroglobin. Subsequently, we have performed the synthesis of these peptides by solid phase methods, and we tested their interaction with neuroglobin by surface plasmon resonance (SPR). Preliminary results confirm unequivocally the specific interaction between synthetic PrP peptides and neuroglobin suggesting a crucial role of PrPC positively charged regions in thisprotein protein association.
Lingua originaleEnglish
pagine (da-a)387-391
Numero di pagine5
RivistaJournal of Peptide Science
Volume17
Stato di pubblicazionePubblicato - 2011

Keywords

  • Surface Plasmon Resonance
  • neuroglobin
  • prion protein

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