The effect of a mild hypothermia (30 degrees C) on sarcoplasmic reticulum (SR) Ca2+ content and release has been evaluated in single cardiac cells loaded with the fluorescent indicator, indo-1. SR Ca2+ content, assessed by rapid caffeine application, is more pronounced at 30 than at 37 degrees C. However, hypothermia reduces the occurrence of spontaneous SR Ca2+ oscillations. In fact, following electrical stimulation, the time to onset of first SR Ca2+ oscillation was increased and their frequency reduced. Since spontaneous SR Ca2+ releases are implicated on the genesis of certain forms of ventricular arrhythmias, the protection provided by a mild hypothermia may be dependent on the modulation of intracellular Ca2+ homeostasis.
|Titolo tradotto del contributo||[Autom. eng. transl.] [Myocardial protection in hypothermia depends on the modulation of intracellular Ca2+ homeostasis]|
|Numero di pagine||3|
|Stato di pubblicazione||Pubblicato - 1992|
- Fluorescent Dyes
- Hypothermia, Induced
- Sarcoplasmic Reticulum