Nutrient availability and nutrient-dependent biochemical signals represent major determinants of cellular senescence and organismal aging. The present chapter describes simple procedures to reliably evaluate the response of cultured cell to nutrients through the longevity protein p66(SHC1) and the mTOR/S6K cascade, which might be used to study cellular senescence and its chemical modulation by pharmaceutical agents in vitro.
- Gene Knockdown Techniques
- HEK293 Cells
- Shc Signaling Adaptor Proteins
- Signal Transduction
- Staining and Labeling